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Table 1 Detection of Methylocella using a functional gene-based PCR assay

From: Facultative methanotrophs are abundant at terrestrial natural gas seeps

Sampling location (country) Coordinates Sampling sub-site (nature of sample, pH) Methane source Methylocella-specific mmoX
Ellicott Creek 1, Amherst (New York, USA) 42.9687 N, 78.7475 W Around main seep (water and sediments, 6.0) Thermogenic natural gas Yes2
Ellicott Creek 2, Amherst (New York, USA) 42.9687 N, 78.7475 W Few meters away from main seep (water and sediments, 6.0) Thermogenic natural gas Yes2
Pipe Creek main seep, West Falls (New York, USA) 42.7042 N, 78.6812 W A large seep with vigorous gas outflow (water, sediments and soil, 6.0) Thermogenic natural gas Yes2
Pipe Creek minor seep, West Falls (New York, USA) 42.7042 N, 78.6812 W A small seep with less vigorous gas outflow (water and sediments, 6.0) Thermogenic natural gas Yes2
Eternal Flame Falls 1, Chestnut Ridge (New York, USA) 42.7014 N, 78.7511 W Main falls (water and sediments, 6.0) Thermogenic natural gas Yes2
Eternal Flame Falls 2, Chestnut Ridge (New York, USA) 42.7014 N, 78.7511 W Pool below the falls (filamentous material and water, 6.0) Thermogenic natural gas Yes
Gasport 1, Gasport (New York, USA) 43.1977 N, 78.5726 W Around minor seeps in Gasport stream (water and sediments, 7.0) Thermogenic natural gas Yes2
Gasport 2, Gasport (New York, USA) 43.1977 N, 78.5726 W Bed of Gasport stream not covered with water (sediments, 7.0) Thermogenic natural gas Yes
Eighteen Mile Creek, N Evans (New York, USA) 42.6963 N, 78.9365 W Edge of the stream (water and sediments, 6.0) Thermogenic natural gas Yes2
Andreiasu Everlasting Fire 1 (Romania) 45.7507 N, 26.8330 E Around gas seep (mud, 8.2) Thermogenic natural gas1 Yes2
Andreiasu Everlasting Fire 2 (Romania) 45.7506 N, 26.8330 E Few meters away from gas seep (dry soil, 8.2) Thermogenic natural gas1 Yes
Beciu mud volcano 1 (Romania) 45.3853 N, 26.7163 E Edge of mud volcanoes (mud, 6.4) Thermogenic natural gas1 Yes2
Beciu mud volcano 2 (Romania) 45.3851 N, 26.7160 E Crater of mud volcanoes (water, 7.2) Thermogenic natural gas1 Yes
Paclele Mari mud volcano 1 (Romania) 45.3396 N, 26.7073 E Edge of mud volcanoes (mud, 8.3) Thermogenic natural gas1 Yes2
Paclele Mari mud volcano 2 (Romania) 45.3395 N, 26.7072 E Edge of mud volcanoes (mud, 8.3) Thermogenic natural gas1 Yes
Paclele Mici mud volcano 1 (Romania) 45.3582 N, 26.7124 E Crater of mud volcanoes (mud, 8.6) Thermogenic natural gas1 Yes2
Paclele Mici mud volcano 2 (Romania) 45.3582 N, 26.7123 E Crater of mud volcanoes (mud, 8.1) Thermogenic natural gas1 Yes
Lakenheath Fen Nature Reserve soil (Thetford, UK) 52.4483 N, 0.5288 E (Peat soil and water, 6.2) Biogenic methane Yes2
Lakenheath Fen Nature Reserve sediments (Thetford, UK) 52.4483 N, 0.5288 E (Sediments and water, 6.5) Biogenic methane Yes2
Moor House Nature Reserve 1 (Pennine Hills, UK) 52.4483 N, 0.5288 E Eroded patches called gullies (peat soil and water, 4.0) Biogenic methane Yes2
Moor House Nature Reserve 2 (Pennine Hills, UK) 52.4483 N, 0.5288 E Non-gullies peat soil (peat soil and water, 4.0) Biogenic methane Yes
Movile Cave microbial mat (Mangalia, Romania) 43.8256 N, 28.5605 E Lake (microbial mat and water, 7.3) Both biogenic and thermogenic Yes2
Movile Cave sediments (Mangalia, Romania) 43.8256 N, 28.5605 E Lake (sediment and water, 7.6) Both biogenic and thermogenic Yes
Movile Cave scrapings (Mangalia, Romania) 43.8256 N, 28.5605 E Air bell walls (soft solid material from walls, 7.3) Both biogenic and thermogenic Yes2
Church Farm soil 1 (Bawburgh, UK) 52.6167 N, 1.1667 E (Soil, 7.0) Biogenic methane No
Church Farm soil 2 (Bawburgh, UK) 52.6167 N, 1.1667 E (Soil, 7.0) Biogenic methane No
Strumpshaw landfill (Norfolk, UK) 52.6027 N, 1.4791 E Soil biofilter from a closed landfill (soil, 7.0) Biogenic methane Yes2
Stiffkey Fen and Salt Marshes 1 (Norfolk, UK) 52.9650 N, 0.9253 E (Soil, 7.0) Biogenic methane No
Stiffkey Fen and Salt Marshes 2 (Norfolk, UK) 52.9650 N, 0.9253 E (Soil, 7.0) Biogenic methane No
Warham Salt Marsh (Norfolk, UK) 52.9617 N, 0.89667 E Sulphur enriched salt marsh (wet soil, 7.2) Biogenic methane No
Warham Salt Marsh (Norfolk, UK) 52.9617 N, 0.89667 E Iron-enriched salt marsh (wet soil, 6.8) Biogenic methane No
  1. 1These sites have been reported to have largely thermogenic natural gas emissions [5, 6] but with potential for biogenic methane [50]
  2. 2Methylocella-specific mmoX verified by the construction of clone libraries from PCR products and sequencing of cloned mmoX fragments