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Table 1 Queen microbiota: results by niche, chronological age, source and biological age

From: The queen’s gut refines with age: longevity phenotypes in a social insect model

Category, group, or OTUa Abundanceb low or high Percent change w/ agec Wilcoxon rank sum testd MANOVAe
F value Pr ≥ F
Mouth
Lactobacillus firm5 H + 59 0.05 ns
*Lactobacillus firm4 L + 195 0.05 ns
Parasaccharibacter apium** H + 341 0.05 ns
Acetobacteraceae Alpha2.1 H − 97 ns 7.8 0.007
Snodgrassella alvi L + 121 0.05 5.6 0.02
Delftia (Burkholderiales) L − 53 0.0009 12.4 0.0008
Midgut
Bifidobacterium asteroides** L + 242 0.03 14.1 0.0004
Lactobacillus kunkeei** H + 336 0.05 5.2 0.03
 *Acetobacteraceae Alpha2.1 L − 79 0.03 ns
*Delftia (Burkholderiales)** L − 93 0.0009 26.2 < 0.0001
Ileum
Bifidobacterium asteroides** H + 164 ns 5.9 0.02
Lactobacillus firm5** H + 9 ns ns
*Lactobacillus kunkeei H + 248 ns 6.4 0.01
Acetobacteraceae Alpha2.1 H − 90 0.005 7.1 0.01
*Parasaccharibacter apium H − 50 0.02 ns
*Snodgrassella alvi H + 279 ns 11.42 0.001
*Gilliamella apicola** L − 69 0.05 ns
Delftia (Burkholderiales)** L − 94 0.0009 36.2 < 0.0001
Rectum
Bifidobacterium asteroides** H + 212 0.005 15.5 0.0002
Lactobacillus firm5 H − 33 0.03 9.3 0.004
Acetobacteraceae Alpha2.1 H − 76 0.009 ns
*Parasaccharibacter apium** H − 78 0.04 11.7 0.001
*Snodgrassella alvi L + 984 0.006 11.4 0.001
*Delftia (Burkholderiales)** L − 92 0.0009 35.7 < 0.0001
  1. aDependent variables are OTUs 1–9 normalized by community size (qPCR) and 16S rRNA gene copy number
  2. bLow abundance (L) = < 1% mean bacterial cell number by niche (Additional file 2: Table S2)
  3. cAverage percent change in bacterial cell number with age. We note that cell number loss cannot exceed 100%
  4. dWilcoxon rank sum test with FDR correction comparing normalized bacterial abundance by chronological age only (Additional file 5: Table S5)
  5. eIndependent variables are queen chronological age and source (df = 3, 59). Reports only F values for chronological age effects examining the top nine most abundant OTUs
  6. *Significant interaction effect of chronological age and source detected by the two-way MANOVA (Additional file 4: Table S4)
  7. **Significant MANCOVA result and Pearson correlation of bacterial abundance with biological age (Additional file 8: Table S8 and Additional file 9: Table S9)
  8. ns not significant