Fig. 2

Weight gain and metabolic markers on a HFD. a, b There were no differences in weight gain between female groups. c, d WT/WT male offspring gained significantly more weight on a HFD than all other groups (repeated-measures two-way ANOVA (time and group) with Tukey’s post-hoc test). e Fat-1/fat-1 displayed the lowest circulating LBP and intestinal permeability (f). Data shown as mean ± SEM. fat-1/WT: n = 8 males, n = 7 females; WT/fat-1: n = 9 males, n = 10 females; fat-1/fat-1: n = 9 males, n = 5 females; WT/WT: n = 10 males, n = 10 females. n = 1–4 animals per cage. Bars with different letters are significantly different. *p < 0.05 and **p < 0.01, WT/WT vs. WT/fat-1; #p < 0.05 and ##p < 0.01 WT/WT vs fat-1/WT; ^&p < 0.05, WT/WT vs. fat-1/fat-1