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Table 5 Selection of Bactrocera tryoni larval midgut isolates cultured and identified by 16S rRNA gene sequencing

From: Near full-length 16S rRNA gene next-generation sequencing revealed Asaia as a common midgut bacterium of wild and domesticated Queensland fruit fly larvae

Isolate

B. tryoni larva number

Source (location)a

Culturing conditionsb

Isolate ID

126

L112

White-fleshed peach (Buxton)

LB O2 37 °C

Enterobacter sp.

132

L117

White-fleshed peach (Buxton)

MRS 5% CO2 37 °C

Leuconostoc sp.

133

L117

White-fleshed peach (Buxton)

MRS 5% CO2 37 °C

Leuconostoc sp.

136

L117

White-fleshed peach (Buxton)

NA O2 25 °C

Enterobacter sp.

155

L117

White-fleshed peach (Buxton)

MRS AnO2 37 °C

Leuconostoc sp.

175

L441

FFPF (Menangle)

MRS O2 30 °C

Asaia sp.

180

L441

FFPF (Menangle)

TSA O2 30 °C

Asaia sp.

182

L441

FFPF (Menangle)

TSA O2 30 °C

Asaia sp.

187

L441

FFPF (Menangle)

GLY pH 5 O2 30 °C

Asaia sp.

191

L441

FFPF (Menangle)

YDC O2 30 °C

Asaia sp.

193

L439

FFPF (Menangle)

TSA O2 30 °C

Asaia sp.

197

L439

FFPF (Menangle)

GLY pH 5 O2 30 °C

Asaia sp.

198

L439

FFPF (Menangle)

YDC O2 30 °C

Asaia sp.

200

L439

FFPF (Menangle)

MRS O2 30 °C

Asaia sp.

  1. aFruit Fly Production Facility (FFPF) in New South Wales, Australia
  2. bMRS de Man, Rogosa and Sharpe agar, TSA tryptone soya agar, YDC yeast dextrose carbonate agar, LB Luria-Bertani agar, GLY pH 5 glycerol-yeast agar, pH 5, AnO 2 anaerobic conditions, O 2 aerobic conditions
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Microbiome

ISSN: 2049-2618

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