Near full-length 16S rRNA gene next-generation sequencing revealed Asaia as a common midgut bacterium of wild and domesticated Queensland fruit fly larvae

Table 4 Nucleotide differences between Asaia V4 region top five unique (100% similarity) OTU representative sequences

		Position	149	162	199	254
Unique representative OTU	Number of sequences	Consensus^a	A	A	A	A
805162	6036
de_novo118	12		N
de_novo341	12				N
de_novo144	11			N
de_novo206	11					N

^aGenerated by aligning the representative sequences of the top unique sequence clusters using only the V4 regions of the near full-length 16S rRNA gene sequences of the dominant Asaia OTU (clustered at 99%)

ISSN: 2049-2618