Near full-length 16S rRNA gene next-generation sequencing revealed Asaia as a common midgut bacterium of wild and domesticated Queensland fruit fly larvae

Table 2 Number of near full-length 16S rRNA gene sequences per larval midgut and 99% OTUs detected

Wild or domesticated	Location; host/diet	Bactrocera tryoni larval ID	Near-full length sequences (> 1300 nt)	OTUs at 99% similarity^a
Wild	Buxton; Peach A	Bux.P.A1	193	3
		Bux.P.A2	329	4
		Bux.P.A3	468	3
	Buxton; Peach B	Bux.P.B1	787	8
		Bux.P.B2	349	8
		Bux.P.B3	59	2
	Buxton; Peach C	Bux.P.C1	6	1
		Bux.P.C2	0	0
		Bux.P.C3	18	1
	Buxton; Peach D	Bux.P.D1	3	1
	Buxton; Peach D	Bux.P.D2	33	4
	Buxton; Peach E	Bux.P.E1	9	1
		Bux.P.E2	2	1
		Bux.P.E3	628	2
	Buxton; Peach F	Bux.P.F1	425	4
		Bux.P.F2	852	3
		Bux.P.F3	639	5
	Buxton; Peach G	Bux.P.G1	190	9
		Bux.P.G2	830	7
		Bux.P.G3	61	5
Wild	Tumut; Peach A	Tum.P.A1	592	9
		Tum.P.A2	121	4
		Tum.P.A3	45	2
	Tumut; Peach B	Tum.P.B1	322	4
		Tum.P.B2	362	2
		Tum.P.B3	371	2
	Tumut; Peach C	Tum.P.C1	211	8
		Tum.P.C2	50	4
		Tum.P.C3	0	0
	Tumut; Peach D	Tum.P.D1	528	3
		Tum.P.D2	126	3
		Tum.P.D3	949	13
	Tumut; Peach E	Tum.P.E1	520	8
		Tum.P.E2	178	7
		Tum.P.E3	360	8
Domesticated	EMAI Fruit Fly Production Facility; artificial carrot diet	FFPF.Col.1	86	3
		FFPF.Col.2	12	3
		FFPF.Col.3	15	2
		FFPF.Col.4	16	3
		FFPF.Col.5	12	1
		FFPF.Col.6	12	2
		FFPF.Col.7	57	2
		FFPF.Col.8	596	2
Domesticated	Gosford Primary Industries Institute; artificial carrot diet	GPII.Col.1	557	2
		GPII.Col.2	100	2
		GPII.Col.3	143	4
		GPII.Col.4	98	1
		GPII.Col.5	21	1
		GPII.Col.6	540	2
		GPII.Col.7	21	1
		GPII.Col.8	26	1
		GPII.Col.9	262	3
Domesticated	Macquarie University; artificial carrot diet	MQ.Col.1	155	1
		MQ.Col.2	243	1
		MQ.Col.3	21	1
		MQ.Col.4	76	3
		MQ.Col.5	109	1
		MQ.Col.6	837	3
	Blank controls	PBS 1 PBS 2 PBS 3	2 0 0	1 0 0
	Positive control	E. coli	0	0
	Total		14,633	195

ISSN: 2049-2618