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Table 1 Classification of 16S rDNA sequences into different taxonomic levels. Numbers in parentheses indicate the number of corresponding operational taxonomic units

From: Hybridization capture reveals microbial diversity missed using current profiling methods

  Hybridization capture Amplicons Shotgun sequencing
Taxonomic level No. of taxa % unassigned sequences No. of taxa % unassigned sequences No. of taxa % unassigned sequences
Domain 2 (354) 0 (0) 1 (115) 0 (0) 1 (13) 0 (0)
Phylum 18 (353) 0.007 (1) 8 (115) 0 (0) 3 (13) 0 (0)
Class 42 (352) 0.014 (2) 16 (115) 0 (0) 5 (13) 0 (0)
Order 73 (338) 0.284 (16) 29 (114) 0.295 (1) 8 (13) 0 (0)
Family 117 (307) 1.260 (47) 35 (112) 0.543 (3) 9 (13) 0 (0)
Genus 143 (263) 4.600 (91) 48 (100) 11.797 (15) 12 (13) 0 (0)
Species 190 (190) 9.419 (164) 73 (73) 29.464 (42) 11 (11) 4.091 (2)