Table 1 Classification of 16S rDNA sequences into different taxonomic levels. Numbers in parentheses indicate the number of corresponding operational taxonomic units
From: Hybridization capture reveals microbial diversity missed using current profiling methods
| Â | Hybridization capture | Amplicons | Shotgun sequencing | |||
|---|---|---|---|---|---|---|
Taxonomic level | No. of taxa | % unassigned sequences | No. of taxa | % unassigned sequences | No. of taxa | % unassigned sequences |
Domain | 2 (354) | 0 (0) | 1 (115) | 0 (0) | 1 (13) | 0 (0) |
Phylum | 18 (353) | 0.007 (1) | 8 (115) | 0 (0) | 3 (13) | 0 (0) |
Class | 42 (352) | 0.014 (2) | 16 (115) | 0 (0) | 5 (13) | 0 (0) |
Order | 73 (338) | 0.284 (16) | 29 (114) | 0.295 (1) | 8 (13) | 0 (0) |
Family | 117 (307) | 1.260 (47) | 35 (112) | 0.543 (3) | 9 (13) | 0 (0) |
Genus | 143 (263) | 4.600 (91) | 48 (100) | 11.797 (15) | 12 (13) | 0 (0) |
Species | 190 (190) | 9.419 (164) | 73 (73) | 29.464 (42) | 11 (11) | 4.091 (2) |