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Table 1 DNA and genome copy numbers measured by the three sampling techniques

From: Filter forensics: microbiota recovery from residential HVAC filters

Sampling technique

Median total DNAa (min-max) (ng/cm 2)

Median bacterial DNAb (min-max) (ng/cm2)

Median fungal DNAb (min-max) (ng/cm2)

Median bacterial genome copiesb (min-max) (GCN/cm2)

Median Fungal Genome copiesb (min-max) (GCN/cm2)

Cut

0.718 (0.638–1.764)

0.02 (0.005–0.119)

0.002 (0.001–0.043)

324 (82–1773)

1 (0.34–19)

Vacuum

4.804 (2.980–12.787)

0.597 (0.311–0.953)

0.180 (0.103–0.341)

15,380 (7901–24,797)

79 (45–150)

Swab

1.523 (0.978–1.898)

0.040 (0.022–0.060)

0.020 (0.010–0.027)

623 (344–916)

9 (4–12)

  1. Note that n = 7 for each of the sampling techniques
  2. aMeasured using Quant-iT™ PicoGreen® dsDNA Assay Kit
  3. bMeasured using qPCR methods