Table 1 Number of reads reported from sequencing a multiplexed viral pathogen reagent
Replicate 1 | Replicate 2 | |||||
|---|---|---|---|---|---|---|
Virus species (most frequent strain) | All 3,505,318 reads | 2,000,000 reads | 150,000 reads | All 2,676,604 reads | 2,000,000 reads | 150,000 reads |
25 target viruses | ||||||
Human astrovirus (Human astrovirus 1) | 27,622 | 15,783.2 | 1184.1 | 8040.6 | 596.2 | 8040.6 |
Enterovirus B (Human coxsackievirus B4) | 27,252 | 15,539.0 | 1161.3 | 22,707.3 | 1704.4 | 22,707.3 |
Human herpesvirus 1 | 67 | 39.0 | 3.5 | 81.4 | 5.5 | 81.4 |
Human herpesvirus 2 | 2 | 1.3 | 0.4 | 7.0 | 0.4 | 7.0 |
Human herpesvirus 3 (Human herpesvirus 3 strain Dumas) | 34 | 17.4 | 1.4 | 84.2 | 6.4 | 84.2 |
Human herpesvirus 4 | 166 | 98.1 | 8.2 | 104.7 | 8.7 | 104.7 |
Human herpesvirus 5 | 8327 | 4743.1 | 352.1 | 7028.5 | 524.4 | 7028.5 |
Human mastadenovirus C (Human adenovirus 2) | 23 | 11.5 | 0.8 | 299.1 | 19.6 | 299.1 |
Human mastadenovirus F (Human adenovirus 41) | 2 | 2.0 | 0.0 | 13.9 | 1.7 | 13.9 |
Human metapneumovirus | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Human parainfluenza virus 1 | 21,387 | 12,170.8 | 921.2 | 9601.2 | 718.6 | 9601.2 |
Human parainfluenza virus 2 | 2879 | 1651.5 | 119.6 | 33.1 | 1.6 | 33.1 |
Human parainfluenza virus 3 | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Human parainfluenza virus 4 (Human parainfluenzavirus 4b) | 21,858 | 12,478.6 | 924.3 | 10,089.8 | 760.2 | 10,089.8 |
Human respiratory syncytial virus | 0 | 0.0 | 0.0 | 229.4 | 15.5 | 229.4 |
Human Rhinovirus A (Human rhinovirus A39) | 0 | 0.0 | 0.0 | 2238.4 | 158.6 | 2238.4 |
Influenzavirus A H1N1 | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Parechovirus A (Human parechovirus 3) | 1,492,756 | 851,560.6 | 63,862.7 | 565,228.5 | 42,434.5 | 565,228.5 |
Rotavirus A | 6 | 2.8 | 0.3 | 8.5 | 0.8 | 8.5 |
Sapporovirus (Sapovirus Hu/GI.2/BR-DF01/BRA/2009 and Hu/G1/BE-HPI01/DE/2012) | 1019 | 575.5 | 45.2 | 62.8 | 5.1 | 62.8 |
Human coronavirus 229E | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Norovirus GI | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Norovirus GII | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Influenza virus B | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Influenza virus A H3N2 | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Non-target viruses | ||||||
Bovine viral diarrhea virus 1 | 1446 | 820.6 | 63.6 | 1891.8 | 140.3 | 1891.8 |
Bovine viral diarrhea virus 2 | 1 | 0.4 | 0.4 | 0.0 | 0.0 | 0.0 |
Primate bocaparvovirus 1 | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Primate bocaparvovirus 2 | 208 | 118.2 | 7.5 | 91.6 | 6.8 | 91.6 |
Human enterovirus (Enterovirus CA55–1988) | 3199 | 1834.7 | 140.6 | 380.6 | 28.7 | 380.6 |
Aichi virus | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Ungulate bocaparvovirus 1 | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Porcine/other circovirus | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Total virus reads | 1,608,254 | 917,448.3 | 68,797.2 | 840,888 | 628,222.4 | 47,138.0 |
Number of detected target viruses | 15 | 14.4 | 11.7 | 17 | 17.0 | 15.7 |