Fig. 5
Ratio of virus reads correlates with concentration of viruses in spiked plasma. Poliovirus/adenovirus and influenzavirus/adenovirus were spiked in healthy donor plasma in different concentrations: at the same concentration for both viruses, ten times more of one virus (keeping the other virus constant) and ten times less of one virus (keeping the other virus constant). The ratio of the sequencing reads for each virus combination was correlated with the concentration ratio (ct value difference) in the input sample. Two independent experiments are shown (circles and triangles, respectively). Shaded areas show the 95% confidence interval. R 2 = 0.74, 0.59, 0.79, and 0.69 and pvalues = 0.04, 0.08, 0.02, and 0.05 for influenza/adenovirus experiments 1 and 2 and polio/adenovirus experiments 1 and 2, respectively