Fig. 5
From: A comprehensive evaluation of the sl1p pipeline for 16S rRNA gene sequencing analysis
Taxonomic assignment is dependent on up-stream choices in 16S rRNA gene processing. sl1p implements 2 methods of taxon assignment across 3 reference databases. By running all methods, we compared taxon assignment against expected control samples. a The negated Bray-Curtis dissimilarity was used to identify which taxonomically assigned OTU sets most closely matched the known composition of the mock HMP communities (a) and the combined URTCul isolates (c). b In a set of 190 single isolate samples, the number of samples whose most abundant OTU correctly matched full-length 16S rRNA Sanger sequencing results is displayed