Fig. 1
![Fig. 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs40168-017-0260-z/MediaObjects/40168_2017_260_Fig1_HTML.gif)
16s rRNA gene data revealed two distinct IBS subgroups, one indistinguishable from healthy controls. a Principal coordinate analysis (PCoA) on the unweighted Unifrac distance matrix from the rarefied data was used to evaluate the presence of clusters or groupings based upon operational taxonomic unit (OTU)-level microbial features. b Hierarchical clustering using average linkage was performed to visualize relationships among the samples based on similarity of microbial composition. Both procedures operate on a phylogenetically informed distance matrix computed using the unweighted UniFrac metric