Table 3 Predicted toxin-antitoxin systems
From: Ancient bacteria of the Ötzi’s microbiome: a genomic tale from the Copper Age
Ancient ORFs | TADB code | TADB gene | Subject | Identity | Aln-len | Mismatch | Gap openings | Q.start | Q.end | S.start | S.end | Log (e value) | Bit score | Predicted annotation |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CADE_0165 | TADB|8353403 | CLL_A3362 | Clostridium botulinum B str. Eklund 17B | 96.6 | 116 | 4 | 0 | 1 | 116 | 1 | 116 | -57.5 | 216.9 | mRNA interferase PemK |
CADE_0166 | – | – | – | – | – | – | – | – | – | – | – | – | – | Hypothetical protein |
CALG_0512 | TADB|8436183 | CLH_2005 | Clostridium botulinum E3 str. Alaska E43 | 76.7 | 146 | 34 | 0 | 1 | 146 | 1 | 146 | −64.3 | 240 | Hypothetical protein |
CALG_0513 | TADB|8436181 | CLH_2004 | Clostridium botulinum E3 str. Alaska E43 | 63.8 | 127 | 46 | 0 | 4 | 130 | 6 | 132 | −45.3 | 176.4 | Hypothetical protein |
CALG_1265 | TADB|375739 | CAC0494 | Clostridium acetobutylicum ATCC 824 | 90.4 | 114 | 11 | 0 | 3 | 116 | 2 | 115 | −54.3 | 206.5 | mRNA interferase PemK |
CALG_1266 | – | – | – | – | – | – | – | – | – | – | – | – | – | CopG family transcriptional regulator |
CPER_1456 | TADB|4710268 | CPF_0812 | Clostridium perfringens ATCC 13124 | 95.8 | 71 | 3 | 0 | 1 | 71 | 1 | 71 | −35.6 | 144.4 | Hypothetical protein |
CPER_1457 | TADB|607725 | CPE0814 | Clostridium perfringens str. 13 | 97.5 | 162 | 4 | 0 | 1 | 162 | 1 | 162 | −87.9 | 318.2 | GNAT family acetyltransferase |
CPER_2012 | TADB|4710831 | CPF_1033 | Clostridium perfringens ATCC 13124 | 99.4 | 168 | 1 | 0 | 1 | 168 | 1 | 168 | −95.5 | 343.6 | Hypothetical protein |
CPER_2013 | TADB|4710829 | CPF_1032 | Clostridium perfringens ATCC 13124 | 100 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | −34 | 139 | DNA-binding protein |
CPER_2208 | TADB|4721113 | CPR_0896 | Clostridium perfringens SM101 | 97.2 | 143 | 4 | 0 | 1 | 143 | 1 | 143 | −77.7 | 284.3 | Hypothetical protein |
CPER_3173 | TADB|4710797 | CPF_1020 | Clostridium perfringens ATCC 13124 | 97.9 | 140 | 3 | 0 | 7 | 146 | 1 | 140 | −75.2 | 275.8 | Hypothetical protein |
CPER_3174 | TADB|4710799 | CPF_1021 | Clostridium perfringens ATCC 13124 | 95.6 | 180 | 8 | 0 | 1 | 180 | 1 | 180 | −93.5 | 337 | XRE family transcriptional regulator |
PFLU_1561 | TADB|9079447 | PFLU2030 | Pseudomonas fluorescens SBW25 | 98.4 | 123 | 2 | 0 | 1 | 123 | 1 | 123 | −64.8 | 241.1 | Hypothetical protein |
PFLU_1562 | TADB|9079443 | PFLU2029 | Pseudomonas fluorescens SBW25 | 88.5 | 113 | 12 | 1 | 1 | 112 | 1 | 113 | −50.8 | 194.9 | Cro/Cl family transcriptional regulator |
PFLU_2731 | – | – | – | – | – | – | – | – | – | – | – | – | – | Hexulose-6-phosphate isomerase |
PFLU_2732 | TADB|348443 | hicB-2 | Pseudomonas putida KT2440 | 71.3 | 108 | 31 | 0 | 5 | 112 | 2 | 109 | −42.4 | 166.8 | Antitoxin HicB |
PFLU_4077 | – | – | – | – | – | – | – | – | – | – | – | – | – | Protein RnfH |
PFLU_4078 | – | – | – | – | – | – | – | – | – | – | – | – | – | Ribosome association toxin RatA |
PVER_0551 | TADB|12012688 | BCAS0580 | Burkholderia cenocepacia J2315 | 55.8 | 129 | 56 | 1 | 1 | 128 | 1 | 129 | −35.9 | 145.2 | Twitching motility protein PilT |
PVER_0552 | – | – | – | – | – | – | – | – | – | – | – | – | – | AbrB family transcriptional regulator |
PVER_1351 | TADB|9070051 | PFLU0443 | Pseudomonas fluorescens SBW25 | 69.9 | 133 | 40 | 0 | 1 | 133 | 1 | 133 | −50.8 | 194.9 | Transcriptional regulator |
PVER_1352 | TADB|9070048 | PFLU0442 | Pseudomonas fluorescens SBW25 | 64.2 | 95 | 34 | 0 | 1 | 95 | 1 | 95 | −33.8 | 138.3 | Hypothetical protein |
PVER_1942 | – | – | – | – | – | – | – | – | – | – | – | – | – | Motility quorum-sensing regulator MqsR |
PVER_1943 | TADB|3146921 | PFL_1052 | Pseudomonas fluorescens Pf-5 | 58.1 | 136 | 55 | 1 | 1 | 136 | 1 | 134 | −38.8 | 155.2 | Transcriptional regulator |
PVER_2079 | TADB|9097595 | PFLU5131 | Pseudomonas fluorescens SBW25 | 94 | 84 | 5 | 0 | 1 | 84 | 19 | 102 | −39.7 | 157.9 | Prevent-host-death protein |
PVER_2080 | TADB|9097599 | PFLU5132 | Pseudomonas fluorescens SBW25 | 95.2 | 84 | 4 | 0 | 1 | 84 | 1 | 84 | −44.9 | 175.3 | Toxin YoeB |
PVER_2187 | – | – | – | – | – | – | – | – | – | – | – | – | – | Addiction module toxin RelE |
PVER_2188 | TADB|9082037 | PFLU2450 | Pseudomonas fluorescens SBW25 | 92 | 100 | 8 | 0 | 1 | 100 | 1 | 100 | −47.6 | 184.1 | Transcriptional regulator |
PVER_2192 | TADB|9082090 | PFLU2458 | Pseudomonas fluorescens SBW25 | 92.8 | 125 | 9 | 0 | 1 | 125 | 1 | 125 | −62.2 | 232.6 | Twitching motility protein PilT |
PVER_2193 | TADB|9082094 | PFLU2459 | Pseudomonas fluorescens SBW25 | 76.9 | 117 | 27 | 0 | 1 | 117 | 1 | 117 | −47.4 | 183.3 | Prevent-host-death protein |
PVER_2252 | TADB|348443 | hicB-2 | Pseudomonas putida KT2440 | 71.3 | 108 | 31 | 0 | 5 | 112 | 2 | 109 | −42.4 | 166.8 | Antitoxin HicB |
PVER_2253 | – | – | – | – | – | – | – | – | – | – | – | – | – | Hexulose-6-phosphate isomerase |
PVER_2785 | TADB|9079443 | PFLU2029 | Pseudomonas fluorescens SBW25 | 88.5 | 113 | 12 | 1 | 1 | 112 | 1 | 113 | −50.8 | 194.9 | Cro/Cl family transcriptional regulator |
PVER_2786 | TADB|9079447 | PFLU2030 | Pseudomonas fluorescens SBW25 | 98.4 | 123 | 2 | 0 | 1 | 123 | 1 | 123 | −64.8 | 241.1 | Hypothetical protein |
PVER_3149 | TADB|3145803 | PFL_0652 | Pseudomonas fluorescens Pf-5 | 78.7 | 94 | 20 | 0 | 1 | 94 | 1 | 94 | −37.3 | 149.8 | Addiction module antitoxin |
PVER_3150 | TADB|3145805 | PFL_0653 | Pseudomonas fluorescens Pf-5 | 59.6 | 99 | 40 | 0 | 1 | 99 | 12 | 110 | −30.3 | 126.7 | Addiction module protein |
PVER_3532 | TADB|9094658 | PFLU4639 | Pseudomonas fluorescens SBW25 | 91.5 | 117 | 10 | 0 | 1 | 117 | 2 | 118 | −57 | 215.3 | Transcriptional regulator |
PVER_3533 | TADB|9094655 | PFLU4638 | Pseudomonas fluorescens SBW25 | 88 | 100 | 12 | 0 | 1 | 100 | 1 | 100 | −46.4 | 180.3 | Toxin RelE |
PVER_4905 | – | – | – | – | – | – | – | – | – | – | – | – | – | Protein RnfH |
PVER_4906 | – | – | – | – | – | – | – | – | – | – | – | – | – | Ribosome association toxin RatA |