Fig. 4

Response of mouse gut metaproteome to high-fat diet feeding. Venn diagrams showing the overlap of identified peptides (a) and protein groups (b) between LFD and HFD groups. The overlap percentage in the bracket was calculated by dividing the overlapped number by the total identified ones for the whole dataset. c PCA score plot. Missing values were imputed with nearest-neighbor method in MATLAB. Numbers in the graph indicate the individual mouse ID in each group. d Heat map of the identified 849 key proteins responding to high-fat feeding. The color of the spot corresponds to the log₁₀-transformed LFQ intensity of each protein. Both column and row clustering were generated based on Euclidean distance using the average linkage method. The sample codes (column) follow the same scheme as in panel (c)