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Table 5 Primers used for 16S rRNA gene amplification of gDNA extracted from stools

From: Characterizing the fecal microbiota of infants with botulism

Fusion PCR Primer Sequencing adapters Library key 16S rRNA Gene target region Target name
A
forward
5’-CCATCTCATCCCTGCGTGTCTCCGAC TCAG CCTACGGGAGGCAGCAG-3’ P1
trP1 forward 5’-CCTCTCTATGGGCAGTCGGTGAT CCTACGGGAGGCAGCAG-3’ P1
A
reverse
5’-CCATCTCATCCCTGCGTGTCTCCGAC TCAG ATTACCGCGGCTGCT-3’ P2
trP1 reverse 5’-CCTCTCTATGGGCAGTCGGTGAT ATTACCGCGGCTGCT-3’ P2