Phylogenetic tree of 16S rRNA genes present in bacterial genomic bins. Top hits from NCBI were included. Many of the White Oak River bacteria are most closely related to sequences recovered from other estuaries and coastal sediments. This tree was generated using the maximum likelihood method in the ARB alignment and phylogeny software package . Closed circles represent maximum likelihood (RAxML, ARB package) bootstraps >75% and open circles are >50% values.