Figure 3
From: A straightforward and efficient analytical pipeline for metaproteome characterization
Pipeline evaluation using 9MM. (A) Analysis of run reproducibility at the ORF level. Left: scatter plot illustrating the correlation between the number of PSMs detected in two runs (r = Pearson correlation coefficient); each point in the plot represents an identified ORF. Right: Venn diagrams depicting the overlap of ORF identifications between the two runs (left diagram, all identified ORFs; right diagram, ORF identified with at least two PSMs; percentage of overlap in brackets). (B) Analysis of run reproducibility at peptide level. Left: scatter plot illustrating the correlation between the number of PSMs detected in the two runs (r = Pearson correlation coefficient); each point in the plot represents an identified peptide. Right: Venn diagrams depicting the overlap of peptide identifications between the two runs (left diagram, all identified peptides; right diagram, peptide identified with at least two PSMs; percentage of overlap in brackets). (C) Quantitative estimation of microbial species abundance according to the number of PSMs identified. For abbreviations of microbial strains, see caption of Figure 1.
