Figure 1

Experimental design. Germ-free mice were inoculated by oral gavage with one of six human inocula. Twenty-one days later (day 0), they received a single intraperitoneal injection of AOM (10 mg/kg). Mice were subsequently administered three five-day rounds of 2% DSS in the drinking water, with 16 days of rest in between. Mice were euthanized 73 days after the AOM injection for enumeration of colonic tumors. The inocula and samples collected on day 0 and day 73 were used for 16S rRNA gene sequencing. AOM, azoxymethane; DSS, dextran sulfate sodium.