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Table 3 Diversity and count estimates of 16S genes in pyrosequencing libraries after sequence trimming

From: An in vitrobiofilm model system maintaining a highly reproducible species and metabolic diversity approaching that of the human oral microbiome

Sample Number of raw 16S rRNA fragment reads Number of OTUs after pre-cluster analysesb Shannon entropy Shannon evenness
Biofilm batch 1 6011 132 3.7436 0.7620
Biofilm batch 2 well 1a 5731 107 3.6971 0.7960
Biofilm batch 2 well 2a 8538 158 4.1868 0.8200
Biofilm batch 3 well 1 4835 154 4.1622 0.8284
Biofilm batch 3 well 2 5166 146 4.1082 0.8289
Biofilm batch 3 well 3 4778 148 4.2334 0.8683
Saliva 1 6956 208 4.7209 0.8544
Saliva 2 6885 243 4.7209 0.8544
Saliva 3 3799 179 4.5234 0.8828
  1. Biofilm batches 1, 2 and 3 correspond to replicate biofilm samples grown at different time points. The biofilm batch-2 library was generated from stable isotope probing experiments (see Methods). aWells 1, 2 and 3 correspond to replicate technical samples collected from different sample wells. bNumbers of operational taxonomical units (OTUs) after precluster-analyses using the SILVA reference alignment and the MOTHUR software.