Intestinal dysbiosis in children with short bowel syndrome is associated with impaired outcome
© Engstrand Lilja et al.; licensee BioMed Central. 2015
Received: 4 March 2015
Accepted: 9 April 2015
Published: 4 May 2015
The composition of the intestinal microbiota seems to be an important factor in determining the clinical outcome in children with short bowel syndrome (SBS). Alterations in the microbiota may result in serious complications such as small bowel bacterial overgrowth (SBBO) and intestinal mucosal inflammation that lead to prolonged parenteral nutrition (PN) dependency with subsequently increased risk of liver failure and sepsis. To date, there are no reported mappings of the intestinal microbiome in children with SBS. Here, we present the first report on the intestinal microbial community profile in children with SBS.
The study includes children diagnosed with SBS in the neonatal period. Healthy siblings served as controls. Fecal samples were collected, and microbial profiles were analyzed by using 16S rRNA gene sequencing on the Illumina MiSeq platform. We observed a pronounced microbial dysbiosis in children with SBS on PN treatment with an increased and totally dominating relative abundance of Enterobacteriacae in four out of five children compared to children with SBS weaned from PN and healthy siblings.
The overall decreased bacterial diversity in children with SBS is consistent with intestinal microbiome mappings in inflammatory bowel diseases such as Crohn’s disease and necrotizing enterocolitis in preterm infants. Our findings indicate that intestinal dysbiosis in children with SBS is associated with prolonged PN dependency.
KeywordsDysbiosis Short bowel syndrome Bacterial diversity Gut microbiota
Pediatric intestinal failure (IF) has been defined as the inability of the gastrointestinal tract to sustain adequate growth, hydration, and electrolyte homeostasis in children without parenteral nutrition (PN). Short bowel syndrome (SBS) is the most common cause of pediatric IF. The condition is caused by massive small bowel resections due to necrotizing enterocolitis (NEC) or volvulus and congenital malformations such as gastroschisis and jejunal atresia. Neonatal SBS is a disease with a high morbidity and mortality . The medical management of SBS aims to establish full enteral/oral feedings and weaning from PN.
The intestinal microbiota seems to be a major factor in determining the successful clinical outcome in SBS defined as independence of PN treatment and intestinal adaptation. Alterations in the microbiota can result in serious complications such as small bowel bacterial overgrowth (SBBO) and intestinal mucosal inflammation that may prevent weaning from PN by compromising intestinal absorptive functions [2,3]. Luminal/oral antibiotic long-term treatment has been recommended for SBBO in children [4,5]. Most cases of SBS occur in neonates during a period when the sterile intestines normally are colonized by bacteria, reaching a microbial profile characteristic of the adult gastrointestinal tract around 2 to 4 years of age . A disruption in the balanced intestinal microbial community, that is, dysbiosis, with an increased relative abundance of facultative anaerobic Enterobacteriaceae in the large bowel is seen in inflammatory bowel disease (IBD) in mouse models, in humans with Crohn’s disease, and in NEC in preterm infants [7,8].
To date, there are no reported mappings of the intestinal microbiota in children with SBS. Here, we present the first report on the microbial profile in children with SBS by using 16S rRNA gene sequencing on the Illumina MiSeq platform.
Characteristics of the study group and corresponding healthy siblings
Remaining small bowel length (cm)
Remaining colon length
Proportion PN of daily nutritional intake
All, (only 2 cm resection)
30 jejunum + 5 ileum
5 jejunum + 17 ileum
45 jejunum + 25 ileum
10 jejunum + 20 ileum
Data collection and statistical analysis
Fecal samples were collected and stored at −80°C until analysis. DNA was extracted from each fecal sample with ultra-clean fecal DNA isolation kit (MoBio, Naxo Ltd, Tartu, Estonia) according to the manufacturer’s instructions.
Sequencing libraries were prepared by amplifying the V3-V4 region of the 16S rRNA gene using the 341f-805r primers, described by Hugerth et al. . After the initial amplification, a second PCR was performed to attach Illumina adapters as well as barcodes that allows for multiplexing. Samples were sequenced using the IlluminaMiSeq, producing in total 10,136,440 2 × 300 base pair reads with an average of 307,165 reads per sample. Primer sequences were trimmed away, and the paired-end reads produced by the sequencing instrument were merged using SeqPrep version 1.1 (https://github.com/jstjohn/SeqPrep) with default parameters and thereafter processed with the QIIME 1.8.0 pipeline (Quantitative Insight into Microbial Ecology) . Merged reads were randomly subsampled to an even depth of 151,610 reads per sample, which was the minimum number of reads among the samples. Using the UCLUST  algorithm built into the QIIME pipeline, sequences were clustered at 97% identity against the Greengenes reference database producing 4,216 OTUs (operational taxonomic units) . For each sample; number of non-singleton OTU as well as most dominant OTU, with respective description, is presented in the supplemental data (Additional file 1). Shannon indexes for diversity were calculated for SBS children on and off PN and tested for significance with Wilcoxon rank-sum test. Using the QIIME pipeline, unweighted UniFrac distances were produced and used for investigation of beta diversity through plotting PCA coordinates. Details on 16S rRNA gene primers, amplification conditions, and sample barcodes are shown in supplemental data (Additional file 2).
We had the unique opportunity to study triplets representing all three groups. Child 2A and child 3A were male triplets born at 23 weeks of gestation. Both boys suffered from NEC in the neonatal period resulting in small bowel resections (Table 1). The third triplet boy (2C2) remained healthy. In child 2A, only 2 cm of the small bowel was resected, however he developed IF after extensive NEC and became dependent on PN. During PN treatment, he had no signs of SBBO. He was weaned to full oral feeding and without antibiotics 3 months before the time of fecal sampling. His intestinal bacterial diversity was similar to his healthy brother (SDI 4, 67 and 4, 97, respectively).
Child 12A was treated with antibiotics only during the first 2 weeks postnatally, had no signs of SBBO, and weaning from PN advanced but slowly. Her fecal bacterial diversity showed Lactobacillacae as the most relative abundant taxonomic family in accordance to our previous findings (Figure 2) . We could detect Clostridium difficile in two out of ten SBS patients (patient 2A and 11A) and in very low relative abundance (data not shown).
The tendency for SBBO and bowel inflammation to delay or prevent weaning from PN in these children with SBS seems to be related to microbial dysbiosis in the intestinal tract. This finding is in accordance with a previous study demonstrating that PN administration was independently associated with SBBO . Influence of PN on the change in profile due to starvation of the microbiome is unlikely since malabsorption of oral/enteral nutrition is the major problem in SBS. In general, the observed changes in the microbiota in SBS children are most likely both at cause and a consequence of the disease status of the child. The limitation of the study is the small study group, and confounding factors that might influence the results are age, intestinal length, and antibiotic treatment. However, the cohort in the present study represents children with SBS in the clinical practice.
In our center, we treat SBBO with oral antibiotics as recommended by other centers [4-6]. However, it is most likely that antibiotics will further contribute to dysbiosis in these children. In children with SBS, normal colonization is disrupted due to early and frequent antibiotic use and decreased bacterial diversity allows potential pathogenic bacteria to expand. Antibiotics have been suggested to lower colonization resistance against Enterobacteriacae such as Escherichia coli and Salmonella enterica, by increasing the inflammatory tone of the intestinal mucosa . Most frequently, probiotics are used to modify the intestinal microbiota in SBS; however, there are conflicting findings and reports of probiotic-associated septicemia .
The overall decreased bacterial diversity in our children with SBS is consistent with intestinal dysbiosis in IBD patients, infants with NEC, and has also been described in a piglet model of SBS [7,8,17,18]. In addition, children with recurrent C. difficile-associated diarrhea show a decreased fecal diversity with a reduction of Bacteriodetes and Firmicutes . In these children, fecal microbiota transplantation (FMT) has a success rate greater than 90%. Such treatment has also been successfully used as a complement to treat IBD [19,20]. Consequently, FMT could prove to be a treatment alternative in carefully selected cases of SBS with dysbiosis. However, since children with SBS often are vulnerable due to their initial health status, the difficulties and risks of FMT must be considered. Although the incidence of severe side-effects is rare, one such risk is contracting illness from the donor where asymptomatic microorganisms that cause no problems in a healthy donor may cause a reaction in the recipient. In addition, mass arrival of a new microbiota may also trigger autoimmune illness as well as bacteria and septic shock. Extra care should also be taken with FMT if the patient has any sign of immunodeficiency .
This is the first report describing the intestinal microbial profile in children with SBS using next-generation sequencing. We observed a pronounced microbial dysbiosis in children with SBS still on PN compared to children weaned from PN with an increased relative abundance of proteobacteria, most of whom has been long-term treated with antibiotics. Our findings indicate that intestinal dysbiosis in children with SBS is associated with impaired outcome with prolonged PN dependency. Future studies need to find out new strategies to treat intestinal dysbiosis in these children.
Availability of supporting data
The data sets supporting the results of this report are included within the article (and its additional file). The sequence data sets are available in the SRA repository, http://www.ncbi.nlm.nih.gov/bioproject/275923.
Fecal microbiota transplantation
Inflammatory bowel disease
Small bowel bacterial overgrowth
Short bowel syndrome
The authors are indebted to research nurse Kajsa Waldenvik for collecting all the samples. This work was supported by the Söderbergs foundation and the Swedish Research Council to LE.
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