Microbiome function predicts amphibian chytridiomycosis disease dynamics

The fungal pathogen Batrachochytrium dendrobatidis (Bd) threatens amphibian biodiversity and ecosystem stability worldwide. Amphibian skin microbial community structure has been linked to the clinical outcome of Bd infections, yet its overall functional importance is poorly understood. Microbiome taxonomic and functional profiles were assessed using high-throughput bacterial 16S rRNA and fungal ITS2 gene sequencing, bacterial shotgun metagenomics and skin mucosal metabolomics. We sampled 56 wild midwife toads (Alytes obstetricans) from montane populations exhibiting Bd epizootic or enzootic disease dynamics. In addition, to assess whether disease-specific microbiome profiles were linked to microbe-mediated protection or Bd-induced perturbation, we performed a laboratory Bd challenge experiment whereby 40 young adult A. obstetricans were exposed to Bd or a control sham infection. We measured temporal changes in the microbiome as well as functional profiles of Bd-exposed and control animals at peak infection. Microbiome community structure and function differed in wild populations based on infection history and in experimental control versus Bd-exposed animals. Bd exposure in the laboratory resulted in dynamic changes in microbiome community structure and functional differences, with infection clearance in all but one infected animal. Sphingobacterium, Stenotrophomonas and an unclassified Commamonadaceae were associated with wild epizootic dynamics and also had reduced abundance in laboratory Bd-exposed animals that cleared infection, indicating a negative association with Bd resistance. This was further supported by microbe-metabolite integration which identified functionally relevant taxa driving disease outcome, of which Sphingobacterium and Bd were most influential in wild epizootic dynamics. The strong correlation between microbial taxonomic community composition and skin metabolome in the laboratory and field is inconsistent with microbial functional redundancy, indicating that differences in microbial taxonomy drive functional variation. Shotgun metagenomic analyses support these findings, with similar disease-associated patterns in beta diversity. Analysis of differentially abundant bacterial genes and pathways indicated that bacterial environmental sensing and Bd resource competition are likely to be important in driving infection outcomes. Bd infection drives altered microbiome taxonomic and functional profiles across laboratory and field environments. Our application of multi-omics analyses in experimental and field settings robustly predicts Bd disease dynamics and identifies novel candidate biomarkers of infection. 6u4HXENqNsd5f8SBedLBdG Video Abstract Video Abstract


Introduction
Amphibians are one of the most vulnerable animal lineages on the planet with over 40% of species threatened with extinction [1]. The pathogenic chytrid fungi Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal) are the causative agents of the skin disease chytridiomycosis [2,3] which is a major driver of global amphibian declines. To date, chytridiomycosis has decimated over 500 amphibian species, representing the greatest loss of biodiversity due to a pathogen ever recorded [4]. Understanding the determinants of chytridiomycosis outbreak dynamics in the wild is therefore necessary to not only control disease spread and mitigate ecosystemlevel impacts [5] but also elucidate the disease ecology of these super-generalist pathogens.
Amphibian skin and its microbiota are the first line of defence against pathogenic invaders [6,7] including Bd [8,9]. Crucially, microbes typically have short generation times, high rates of mutation and large within-host population sizes that facilitate faster adaptation of defensive traits than is possible in long-lived hosts [10]. Host-associated microbes may provide protection against infection by outcompeting pathogens for resources [11], producing antimicrobial compounds [12][13][14], priming the host immune system [15] and in some instances preying upon pathogens [16]. Prior studies have identified bacterialderived antimicrobial compounds that inhibit Bd growth [12-14, 17, 18]; however, determining overall microbiome community function remains challenging due to the complex web of interactions occurring amongst microbes [17,19]. Microbial taxa can also switch from being inhibitory to promoting pathogen growth based on community composition [19] and in more extreme cases can shift from being beneficial to parasitic (or vice-versa) in processes that may be mediated by host factors [20,21], the abiotic environment [22] or microbial community ecology [23]. As well as their role in defence, microbial communities are important in maintaining host dermal homeostasis [24][25][26][27]. Disruption to microbial community function and microbe-host co-metabolic pathways (often driven by pathogen invasion) can consequently negatively impact host health [28][29][30][31][32]. Microbiome function therefore cannot be predicted by taxonomy alone and studies need to integrate functional inter-relationships amongst microbes and their hosts.
We combine omics methods to determine how amphibian skin microbial community structure and function shape chytridiomycosis disease dynamics in a wild outbreak and laboratory in vivo infection model.
Our wild system centres on midwife toad (Alytes obstetricans) populations in the French and Spanish Pyrenean mountains that present epizootic or enzootic disease dynamics based on long-term observations of sustained Bd-induced population decline or recovery respectively [33]. A prior study of the Pyrenean system demonstrated no clear link between pathogen genetics and epidemiological trends, but a strong association between bacterial community structure and disease dynamics [33]. The functional relevance of these bacterial community differences with relation to disease is however not known.
We show that cross-kingdom microbial communities are associated with Bd infection dynamics in the wild and that Bd clearance in the laboratory is associated with an altered microbiome state. Further, microbiome function maps to taxonomic beta diversity in both the laboratory and field, exhibiting a disease-associated profile. These findings indicate that taxa differences predict differences in function and are therefore not consistent with functional redundancy.

Ethics statement
Fieldwork was performed under licence from the Parc National des Pyrénées (2016-110 and 111) and the Instituto Aragonés de Gestión Ambiental. Animal experiments were carried out in accordance with The Animals (Scientific Procedures) Act of 1986 Directive 2010/63/ EU and followed all codes of practice which reinforce this law.

Field sampling
This study focussed on four Alytes obstetricans populations in the French and Spanish Pyrenees (SI Fig. 1, SI Table 1) that are part of a long-term Bd surveillance project [33]. In recent years, three populations (Ibon Acherito, Puits d' Arious and Lhurs) have exhibited low Bd infection intensity and stable population abundance consistent with the development of Bd resistance and enzootic disease dynamics. Conversely, Arlet is typified by epizootic disease dynamics based on continued population decline since the outbreak of Bd in 2005, high Bd infection loads and perennially high mortality rates [33]. We sampled fourteen A. obstetricans metamorphs for Bd, skin microbes and metabolome from each population (n = 56) in August 2016. New nitrile gloves were worn for each animal. Animals were rinsed with sterile water to remove transient microbes/substrate debris; then, microbial communities and Bd were each sampled Keywords: Microbiome, Metabolome, Multi-omics, Amphibian, Batrachochytrium dendrobatidis, Chytridiomycosis using a single sterile MW100 cotton dry tipped swab (MWE Medical Wire, Corsham, UK) that was rolled over the ventral and dorsal surfaces of the skin ten times, and hindlimbs five times. To collect skin mucosal metabolome, each animal was placed in a falcon tube containing 12 ml of sterile water for 20 min to allow metabolites to diffuse into solution. Metabolites were collected using solid phase extraction (Supplementary methods).

Experimental infection
Forty adult A. obstetricans bred in captivity from wildcaught animals from Pyrenean populations (Arlet, Ansabere and Lhurs) were randomly assigned to 1.6L plastic enclosures containing an autoclaved damp paper towel and a plastic cover object and were fed crickets (Acheta domesticus) ad libitum twice weekly. Animals were housed individually for two weeks prior to the experiment to allow acclimatisation to experimental conditions. Enclosures were cleaned twice weekly with Rely+ on Virkon (Antect International Ltd., Suffolk, UK). The experimental facility was kept on a 12h light/dark cycle and maintained at 18°C. Animals were swabbed prior to Bd exposure on day 1 and on days 30 and 60 post-exposure. Twenty animals were repeatedly exposed on days 1, 3, 5, 7, 9 and 11 to 2 ml of 1.5 × 10 4 Bd zoospores for 4 h with the remaining 20 control animals being exposed to a sham infection containing only nutrient media (mTGHL). On day 30, metabolome samples were collected from each animal following the same protocol as field samples. On day 60, all animals were euthanised.

Sample processing/analysis
Microbial genomic DNA was extracted from swabs using the DNeasy Blood and Tissue kit (Qiagen, Venlo, Netherlands) according to the manufacturer's instructions. A mutanolysin pre-treatment was included to enhance bacterial DNA recovery [34]. We amplified the V4 region of the 16S rRNA gene and the ITS2 region of the fungal internal transcribed spacer (ITS) using custom barcoded primers and PCR conditions adapted from a prior study [35] (Supplementary methods). 16S and ITS2 data was analysed using DADA2 [36] and our previously published method [31] respectively (Supplementary methods).
Bd DNA was extracted using a bead-beating protocol [37]. Extractions were diluted 1/10 before undergoing qPCR amplification with samples run in duplicate [37] and with Bd standards of 100, 10, 1 and 0.1 zoospore genomic equivalents (GE). Samples with greater than 0.1 GE were considered Bd positive.
Metabolome samples were analysed in a randomised order by ultra-high-pressure liquid chromatographymass spectrometry (UHPLC-MS) (Supplementary methods).
Shotgun metagenome sequencing was performed using an Illumina HiSeq 2000 on a subset of samples from the field study (n = 50) and day 30 of the experiment (n = 20). DNA sequences were merged, quality checked and annotated using the Metagenomics Rapid Annotation (MG-RAST) server (vs. 4.0.3.) with default parameters [38]. Taxonomic analysis was performed using the RefSeq database with the following cut-off parameters: e-value of 1E-5, minimum identity of 60%, maximum alignment length of 15 bp and constrained to only bacterial reads. Functional analyses of bacteria were performed using the KEGG subsystem database.

Bd infection analysis
Bd genomic equivalents data was log 10 +1 transformed to fit a Gaussian distribution and analysed using ANOVA. A Tukey's post-hoc test was performed to identify statistically significant differences between populations.

Alpha diversity
Bacterial reads per sample for the laboratory and field study had a mean of 27011 (sd = 10448) and 22830 (sd = 10378) respectively. Fungal sequences per sample for the field and laboratory study had a mean of 731 (sd = 1183) and 2570 (sd = 5668) respectively. To mitigate the effects of uneven sampling [39] bacterial field and laboratory samples were rarefied to 3658 and 10692 sequences respectively, corresponding to the depth of the lowest read samples. Fungal samples for the field study were rarefied to 233 reads per sample resulting in 35 samples out of 56 of the sequenced samples remaining for downstream analysis. For the laboratory challenge experiment, fungal samples were rarefied to 200 reads per sample resulting in 84 samples remaining. To examine whether bacterial and fungal Shannon diversity differed across populations, we performed ANOVA and Tukey's posthoc test. For the 16S data, Shannon diversity was log transformed to improve fit to a Gaussian distribution. For our experiment, we performed a non-parametric Mann-Whitney test for each time point to compare Shannon diversity in the control and Bd-exposed treatment.
Shotgun metagenomics functional KO (KEGG Orthology) data ranged from 2101 to 1,344,436 KO reads per sample for the field data and 812,729 to 2,410,428 KO reads per sample for the laboratory data. We rarefied our data to 9847 and 812,729 KO reads for our field (leaving 47 samples) and laboratory data respectively. Differences in Shannon diversity between populations in the field and Bd exposure in the laboratory were assessed using ANOVA and a t-test respectively.

Beta diversity and differential abundance
Fungal taxonomic data was filtered to remove samples with fewer than 233 and 200 sequences for the field and laboratory studies respectively. For shotgun metagenomic field data, we filtered samples with fewer than 9000 KO reads resulting in 47 samples remaining. Bacterial 16S and shotgun data were subsequently filtered to include features with a mean relative abundance > 0.01% and fungal data was filtered to include taxa with a relative abundance > 0.05%. Zero values attributed to rare taxa/features were replaced using the Bayesian "CZM" method using the zCompositions R package [40] before centred-log ratio (CLR) transformation. To determine if beta diversity of 16S, ITS2, shotgun and metabolome data differed based on population and disease state in the wild, or Bd treatment in the laboratory, each dataset was visualised using principal components analysis (PCA) using mixomics [41]. To quantify differences in beta diversity based on host population and disease dynamic (wild) or day, Bd and their interaction (laboratory), we applied permutational multivariate analysis of variance (PERMANOVA) on Euclidean distances using the adonis function in the R package vegan [42] with 999 permutations.
To identify ASVs/OTUs/KOs driving differences in beta diversity based on wild disease dynamic or on experimental treatment on day 30 (the time point corresponding to peak Bd infection), we performed sparse partial least squares-discriminant analysis (sPLS-DA) [43] using mixomics [41]. We tuned our sPLS-DA models (function tune.splsda, mixomics package) using 10 × 5-fold cross-validation to determine optimal model parameters (number of components and feature selection). The performance of our sPLS-DA models were assessed using 100 × 5-fold cross-validation. We further identified ASVs/OTUs/KOs that differed in abundance in the epizootic and enzootic populations or experimental treatment for days 1, 30 and 60 of the experiment using ALDEx2 [44] and selected informative features based on Benjamini-Hochberg (BH) corrected Welch's t test p-values < 0.05 and an effect size 1> and < − 1.
To assess the congruency of taxonomic data generated from shotgun metagenomics and the bacterial taxonomic 16S data in the field, we performed Procrustes analysis on Euclidean distances of RefSeq CLR-transformed abundance and CLR-transformed 16S data using the R package vegan [42]. The PROTEST function was used which performs repeated symmetric analyses to estimate if the degree of association of the two matrices is greater than that expected by chance alone.
Wilcoxon's tests were performed to determine differentially abundant metabolites based on disease dynamic (wild) or Bd exposure (laboratory). P-values were adjusted using the false discovery rate procedure and significant features that also had a log2 fold change > 1.5 were considered most informative as possible biomarkers. Partial least squares discriminant analysis (PLS-DA) was performed using mixomics [41]. The PLS-DA model was assessed using 5-fold cross-validation repeated 50 times and model significance was tested using a permutation analysis (999) implemented using MVA.test in the RVAideMemoire package [45]. Three components were selected for PLS-DA for field data (NMC = 0.02, p = 0.001) and laboratory data (NMC = 0.078, p = 0.001). Metabolites driving differences in metabolome variation based on wild disease dynamic or laboratory pathogen exposure were identified based on the variable influence on the projection (VIP) parameter on component 1. The most informative metabolites were determined based on a VIP score > 2 and that also showed a statistically significant profile at q < 0.05 and log 2 fold change > 1.5 from the univariate analysis.

Omics integration
To determine the degree of association between bacterial/fungal taxonomic community structure and overall metabolite composition, we performed Procrustes analysis on Euclidean distance matrices using the vegan package [42]. To determine if a multi-omics signature could differentiate enzootic/epizootic disease dynamics or laboratory Bd exposure on day 30 for bacteria and metabolite data, we applied the supervised method, data integration analysis for biomarker discovery using latent components (DIABLO) [46]. We used the CLR-transformed microbiome data (filtered to the top 0.01% of bacterial ASVs) and g-log-transformed metabolite data as inputs. The block link was set to 0.1. Model parameterisation (perf function) was performed to select the number of components to use in our final models. A tuning procedure (tune.block.splsda) with 50 × 10-fold crossvalidation was applied to determine the optimal number of variates. Final model performance was assessed using 10-fold cross-validation repeated 50 times. We did not apply supervised analysis to our fungal data due to poor model fit.
To cross-validate our supervised analysis and also examine fungi-metabolite associations in the field, we applied an unsupervised omics integration methodsparse partial least squares (sPLS) regression, using the mixomics package in R [41]. For model parameterisation, we set the number of metabolite features to include in our model for each component (keepY) to 111, corresponding to the number of metabolites found to be discriminative of disease dynamic based on single omics analyses. We selected 21 bacterial ASVs to be included for each component (keepX), which corresponds to the number of differentially abundant taxa with an effect size > 1 or < − 1 and corrected p-values < 0.05 as identified from ALDEx2 analysis based on disease dynamic. For wild fungal-metabolite interactions, we used a keepX of 10 given the low number of differentially abundant fungal OTUs. We visualised microbe-metabolite associations with a correlation co-efficient > 0.65 using relevance networks plotted in cytoscape [47].
We report shared features recovered from single omics and DIABLO analyses for the laboratory experiment and field study. We identified metabolite features with identical KEGG annotations and therefore are more likely to be the same metabolite. For bacterial taxa identified as discriminatory in the laboratory and field studies, we performed Spearman's correlation of CLR-transformed taxa abundance and log 10 +1 GE load for day 30 experimental data.

Host skin bacterial and fungal taxonomic composition is associated with disease dynamic and population
Bacterial Shannon diversity differed based on population (ANOVA F (3,52) = 8.58, p < 0.001), with Acherito being significantly higher than Arlet (p < 0.001) and Lhurs (p < 0.001). Fungal Shannon diversity did not differ by population (p > 0.05). Disease dynamic and population varied in beta diversity of bacteria and fungi (Bacteria: disease dynamic PERMANOVA Pseudo-F (1,54) Fig. 1b, c).
Twenty-nine bacterial ASVs were associated with epizootic dynamics and a single ASV was associated with enzootic populations from sPLS-DA (Fig. 1d, Supplementary Data 1). Nine fungal OTUs were associated with epizootic dynamics on sPLS-DA component 1 (Fig. 1e), whilst component two had 22 and 8 OTUs linked to enzootic and epizootic dynamics respectively (Supplementary Data 2). Seven out of nine fungal OTUs on component 1 were classified as Batrachochytrium (Fig. 1e), whilst OTUs on component 2 belonged to eight classified fungal classes (Supplementary Data 2). Differential abundance analysis using ALDEx2 identified 21 bacterial biomarkers for disease state, of which 1 and 20 were associated with enzootic and epizootic disease dynamics respectively (Supplementary Data 3). Discriminatory bacterial taxa spanned four phyla and seven classes. For the enzootic populations, the single discriminatory ASV (also identified using sPLS-DA) was assigned to the Massilia genus (order: Burkholderiales), whilst ASVs in the epizootic population belonged to the Sphingobacteriales, Actinomycetales, Burkholderiales, Chlamydiales, Flavobacteriales, Rhizobiales and Xanthomonadales. ALDEx2 identified one fungal OTU (Batrachochytrium sp.) significantly associated with epizootic dynamics (Supplementary Data 4).

Host bacterial community function associates with disease dynamic and population
Procrustes analysis showed significant correlation between shotgun bacterial taxonomic data and 16S data (Procrustes m12 squared = 0.43, correlation in symmetric Procrustes rotation = 0.76, p = 0.001). Shannon diversity of KO hits differed based on population (ANOVA F (3, 43) = 9.56, p < 0.001) with Acherito being significantly higher than Arlet (p < 0.001) and Lhurs (p < 0.001), whilst Puits was also higher than Arlet (p = 0.03). KO beta diversity differed based on disease dynamic and population (disease dynamic PERMANOVA Pseudo-F (1, 45) = 6.80, R 2 = 0.13, p = 0.001, population PERMANOVA Pseudo-F (3,43) = 4.50, R 2 = 0.24, p = 0.001, Fig. 2a-d). Analysis of bacterial KOs using sPLS-DA found the main sources of variation was in amino acid metabolism, carbohydrate metabolism and energy metabolism (Fig. 2e, Supplementary Data 5 Fig. 3a). Univariate analysis identified 870 differentially abundant metabolite features, of which 183 had a log 2 fold change > 1.5 (Fig. 3b, Supplementary Data 7). PLS-DA based on disease dynamic yielded 121 metabolite features with a VIP score > 2, of which 111 were considered most informative (VIP > 2, q < 0.05, log 2 fold change > 1.5, Supplementary Data 8). Following putative annotation of the most informative metabolite features, one of the features was suspected to be the anti-Bd metabolite indole-3-carboxaldehyde. More detailed investigation of the MS/MS data for this metabolite (m/z 144.04606 RT 6.96 min) kindled our interest in a second, following the peak of the same m/z in the spectra at 8.73 min, present in the XCMS matrix but originally filtered out as thought to be due to some higher blank signal. However, upon closer inspection, the intensities of this second peak behaved similarly to m/z 144.04606 with higher intensity in the epizootic population (Wilcoxon's test W = 17, p < 0.001, SI Fig. 2). Whilst both ions were putatively annotated as the anti-Bd metabolite indole-3-carboxaldehyde, the MS/MS data for the second signal resembled those in spectral databases (https:// mona. fiehn lab. ucdav is. edu/ spect ra/ displ ay/ PR100 508; https:// mona. fiehn lab. ucdav is. edu/ spect ra/ displ ay/ PB000 507) and from an authentic standard fragmented in-house, thus providing evidence for the presence (and increased abundance in the epizootic population) of indole-3-carboxaldehyde on wild A. obstetricans skin.

Microbiome/metabolome interactions in the wild
Procrustes analysis showed a significant association between bacterial/fungal taxonomy and metabolic profile (bacteria: m12 square = 0.45, correlation in symmetric Procrustes rotation = 0.74, p = 0.001; fungi: m12 square = 0.60, correlation in symmetric Procrustes rotation = 0.63, p = 0.004). DIABLO confirmed enrichment of eight bacterial ASVs in the epizootic population that were also identified from a single omics analysis. Of the five informative metabolites recovered from our analysis, three were indicators of epizootic disease dynamics and one was linked to enzootic dynamics (Wilcoxon's test q < 0.05, log2 fold change > 1.5, Supplementary Data 9). Network analysis identified a single cluster representative of epizootic dynamics based on the presence of epizootic discriminatory taxa and their positive correlations with the epizootic enriched metabolites (Fig. 3c). Meanwhile, negative correlations were found between epizootic taxa and the single metabolite feature that was enriched in the enzootic populations. Unsupervised sPLS regression supports findings from supervised analysis with 31 of the 40 bacteria-metabolite pairs from DIABLO also recovered in sPLS. We identified 450 correlated pairs of bacterial ASVs/metabolite features (39 negative, 411 positive) involving 20 ASVs and 157 metabolite features forming two sub-networks (SI Fig 3a,b, Supplementary Data 10). Bacterial sub-network A was reflective of epizootic dynamics based on a dominance of epizootic discriminative ASVs that were positively correlated with epizootic enriched metabolite features. Sphingobacterium had the greatest betweenness centrality (a measure of how often a node occurs on all shortest paths between two other nodes and therefore an indicator of functional importance). In bacterial sub-network B, an ASV belonging to the Sinobacteraceae family and Haliscomenobacter exhibited the greatest betweenness centrality.
Fungal-metabolome analysis also yielded two subnetworks comprising 461 fungal-metabolite pairs (152 negative, 309 positive) involving 11 fungal OTUs and 109 metabolite features (SI Fig 4a,b, Supplementary Data 11). Fungal sub-network A comprised 8 OTUs, all of which were discriminant for epizootic dynamics from sPLS-DA, with ALDEx2 also identifying one OTU (sp633) as discriminant for epizootic dynamics. Fungal sub-network A was dominated by chytrid with 7 OTUs classified as Batrachochytrium sp. Twenty-seven metabolite features in fungal sub-network A were positively correlated with fungal taxa that were discriminative for epizootic dynamics, whilst twenty-three metabolite features were negatively correlated with fungal taxa in subnetwork A that were discriminative for enzootic dynamics. Fungal subnetwork B comprised three OTUs-two belonging to the Massarinaceae and an unknown Dothidiomycete.

Experimental infection alters skin microbiome state
Nineteen out of twenty Bd-exposed animals became infected with Bd by day 30, with Bd infection intensity ranging from 0.88 to 427.08 genomic equivalents (GE) (SI Fig 5). On day 60, a single Bd-exposed animal remained infected, with all other animals clearing infection. There was no mortality.
We show no effect of Bd exposure on bacterial Shannon diversity at any time point measured (p > 0.05). Fungal Shannon diversity was reduced in Bd-exposed animals compared to the control group on day 30 of the experiment (W = 109, p = 0.005) but not on day 0 or 60 (p>0.05). We confirm no significant difference in beta diversity for bacteria and fungi at the start of the experiment (PERMANOVA p > 0.05). Beta diversity differed by day, Bd exposure and their interaction for bacteria (PER-MANOVA day F (1,116) Fig. 4a, b). Twenty bacterial ASVs and two fungal OTUs were found to drive differences in beta diversity on day 30 according to sPLS-DA (Supplementary Data 12, 13). The bacterial ASVs spanned three phyla and eight classes, whilst fungal OTUs all belonged to the Basidiomycete phylum and the Class Tremellomycetes. ALDEx2 found no differentially abundant bacterial or fungal taxa at day 0. At day 30, three bacteria and one fungal taxa differed between treatments (Supplementary Data 14, 15). Sphingobacterium and Acinetobacter were associated with the control group, whilst Comomonas was increased in the Bd exposure group. The fungus Trichosporon had significantly higher abundance in the control group compared to Bd-exposed animals on day 30. For day 60, we identified three discriminatory taxa (Comamonas, Brevundimonas and Gemmobacter, Supplementary Data 16), all of which were associated with Bd exposure. No fungal taxa were differentially abundant at day 60.
KO Shannon diversity at day 30 was lower in the Bdexposed group than the control (t-test df = 17.43, t = 2.96, p = 0.009). KO beta diversity differed by Bd exposure (PERMANOVA F (1,18) Fig. 5a-c). Forty discriminatory KOs were identified from sPLS-DA (Fig. 5d, Supplementary Data 17). ALDEx2 identified 303 discriminatory KOs, with 114 and 189 increased in the control and Bd-exposed groups respectively (Supplementary Data 18). Differentially abundant KO hits were mostly associated with amino acid metabolism, carbohydrate metabolism, membrane transport, signal transduction and metabolism of cofactors and vitamins.
UHPLC-MS metabolomics resulted in a peak list with 2329 features. Metabolome differed based on Bd exposure (PERMANOVA F (1, 38) = 4.50, R 2 = 0.11, p = 0.003). Wilcoxon's tests identified 729 features of which 37 had a log 2 fold change > 1.5 and q < 0.05 (Supplementary Data 19). PLS-DA produced separation based on Bd exposure with 111 metabolite features having a VIP score > 2, of which 24 had a log 2 fold change > 1.5 and q < 0.05 (Supplementary Data 20). Screening for anti-Bd metabolites found an increased abundance in the Bd-exposed group of a metabolite feature putatively annotated as indole-3-carboxaldehyde (q < 0.05).
Microbiome/metabolome integration using DIABLO [48] identified bacterial and metabolite features discriminating control and exposure treatments (Fig. 6a,b,  Supplementary Data 21). Bd exposure was associated with enrichment of bacterial ASVs and metabolite features (Fig. 6c, d) indicating that infection drives changes in microbe-metabolite interactions. All three discriminatory bacterial ASVs (Acinetobacter, Sphingobacterium, Comamonas) identified using ALDEx2 were also identified by DIABLO.

Biomarkers in the laboratory and field
We identified common biomarkers in the laboratory and field for bacterial 16S, shotgun and metabolome, but not fungal ITS2 data (Supplementary Data 22). Three bacterial ASVs (Sphingobacterium, Stenotrophomonas, Comamonadaceae) were discriminatory in the laboratory and field, with increased abundance in the epizootic population but reduced abundance in the Bd-exposed experimental group (in which all animals survived), indicating an association with negative health outcomes. Of these taxa, Stenotrophomonas abundance was significantly positively correlated with Bd infection intensity on day 30 of the experiment (SI Fig 6). Eighteen bacterial KOs were discriminatory in both the laboratory and field. Six metabolite annotations from the laboratory and field were identical including indole-3-carboxaldehyde, a bacterial-derived anti-Bd metabolite [12] that was increased in the epizootic population and in the Bd-exposed laboratory treatment.

Discussion
We show that bacterial and fungal taxonomy, bacterial functional profile and skin metabolome reflect Bd disease dynamics in the wild and exposure in the laboratory. Batrachochytrium clearance coinciding with an altered microbiome state in laboratory animals supports prior findings [30,31,48], but here suggests a non-detrimental or even protective microbiome akin to wild enzootic populations. Conversely, the distinct microbiome community structure and function of the epizootic population suggests a deleterious relationship with amphibian health in the context of infection [30,31].
The existence of a detrimental microbiome profile with epizootic dynamics is further supported by the high relative abundance of taxa with known positive associations with Bd, or that have been associated with co-infections in amphibians. Of note are Sphingobacterium, Rhodococcus, Parachlamydia, Chryseobacterium, Flavobacterium, Acinetobacter and Stenotrophomonas, which were identified as indicator taxa of epizootic dynamics in the previous year [33] and are known to contribute to amphibian ill health or promote Bd growth [30,31,[49][50][51][52][53]. Three bacterial ASVs (Sphingobacterium, Stenotrophomonas and an unclassified Commamonadaceae) were discriminatory for epizootic dynamics in the field but associated with the control group of the experiment, indicating that high abundance of these taxa is linked with poor infection outcomes. Spearman's correlation revealed a positive relationship between taxa abundance and Bd infection at day 30 of the experiment for Stenotrophomonas (significant) and Sphingobacterium (non-significant), further indicating a synergistic relationship between these taxa and Bd.
Batrachochytrium sp. were also the dominant fungal taxa associated with epizootic disease dynamics, indicating that high Bd infection load is associated with , Bd exposed = 20 (each sample day). Sample sizes fungi: day 1 control = 9, day 1 exposed = 12, day 30 exposed = 16, day 30 control = 8, day 60 exposed = 19, day 60 control = 20 microbiome differences. A single bacterium identified as Massilia had increased abundance in the enzootic population and is known to be Bd inhibitory in the laboratory [52], producing the Bd inhibitory metabolite violacein [54]. This finding suggests that bacterial taxa other than Janthinobacterium lividum reside on the amphibian skin and may confer similar protective benefits, further highlighting the importance of considering bacterial function as well as taxonomy. Given the opposite patterns of discriminate taxa abundance in animals that cleared infection in the laboratory and from the wild epizootic population, our findings suggest that Bd resistance is driven by stemming proliferation of certain detrimental bacteria coinciding with Bd colonisation and that protection may therefore be better predicted by broader aspects of community ecology.
Skin microbiome function also differed based on Bd exposure/disease dynamics, as demonstrated by disease-associated patterns in beta diversity for shotgun metagenomic and metabolomic profiles. Specifically, we found disease-associated differences in bacterial metabolism of amino acids, carbohydrates, glycans, terpenoids and polyketides, vitamins and cofactors and energy metabolism. We also found differences in bacterial environmental information processing suggesting that Bd infection results in changes to both the metabolic environment and bacterial interactions.
Bacterial gene abundance in wild enzootic populations and laboratory Bd-exposed animals supports possible microbe-mediated protection through enrichment of genes of potential importance in bacteria-Bd competition. For example, arginine decarboxylase and ornithine decarboxylase cause a dose-dependent reduction of Bd growth up to 84% when blocked in vitro [55] and were increased in Bd-exposed laboratory animals and wild enzootic populations respectively. In laboratory Bd-exposed animals, we also find evidence for zinc competition through increased abundance of the bacterial genes znuA (involved in environmental zinc uptake) and superoxide dismutase (SOD1), which uses a zinc cofactor. Bacterial competition for zinc provides a novel theoretical mechanism of Bd growth limitation and virulence attenuation, since zinc is required by Bd virulence factors (m36 metalloproteases) [56,57]. An increased abundance of bacterial chitin deacetylase, which breaks down the fungal cell wall component chitin [58] into the antifungal agent chitosan [59] was also found in laboratory Bdexposed animals and may be a bacterial mechanism of Bd inhibition.
Bacterial environmental information processing and sensing was identified as a key factor differentiating Bd infection/disease dynamics. Specifically, genes involved in the bacterial two-component system (TCS) exhibited increased abundance in the enzootic populations and the Bd-exposed treatment in the laboratory. The TCS plays an important role in bacterial sensing and adaptation to environmental stimuli [60] where it can modulate bacterial virulence [61], antimicrobial resistance [62], environmental stress [63,64], biofilm production [65] and is a key component in the establishment of symbiosis [66,67]. In our laboratory experiment, we identified a large number of differentially abundant genes from the ATPbinding cassette (ABC) transporter family, which can be regulated by the TCS [68]. Differential abundance of TCS and ABC transporters with Bd infection is likely a response to changes in the skin molecular environment through both host and Bd derived metabolites. Of particular importance may be TCS-mediated resistance to antimicrobial peptides (AMPs) via ABCs transporters [69]. Alytes obstetricans is well known for its production of AMPs and toxins by skin serous glands [70]. Therefore, living in concert with this antimicrobial arsenal is critical to the survival of beneficial amphibian skin-associated bacteria in order to colonise, persist and work mutualistically with host defences. We find evidence for this in the laboratory Bd-exposed treatment, which showed increased abundance of genes involved in the yejABEF microcin C transporter system that confers resistance to AMPs in other host models [71].
Putative bacterial-produced Bd inhibitory metabolites were also found in both the laboratory and field, with the anti-Bd compound indole-3-carboxaldehyde discovered as a biomarker for laboratory Bd exposure and wild epizootic dynamics. Janthinobacterium (an indole-3-carboxaldehyde producer) [13] was also present in both the laboratory and field (although did not significantly differ in abundance) as were bacterial genes from the tryptophan pathway (in which indole-3-carboxaldehyde is a metabolic product). The increased abundance of putative indole-3-carboxaldehyde in the epizootic population may indicate that anti-Bd metabolites alone are not enough to counter infection and that indole-3-carboxaldehyde production may be Bd dose dependent.
We found strong correlations between microbial taxonomy and skin metabolome. This likely reflects hostderived metabolites shaping microbiome structure and microbe-derived metabolites contributing to the skin metabolome. Microbiome and metabolome integration Fig. 6 Integration of skin bacterial microbiome and metabolome identifies a Bd infection-associated multi-omics signature. DIABLO sample plots demonstrating discrimination of Bd-exposed and un-exposed midwife toads based on a) skin bacterial microbiome and b) skin metabolome c) bacterial taxa contributing separation along component 1 in (a). Bar length indicates loading coefficient weight of selected bacterial ASVs. Bar colour indicates the group in which the bacterial ASV has the highest median abundance, blue = control, red = Bd exposed. recovered discriminatory taxa and metabolite features that were also identified from single omics analyses, providing confidence in their classification as disease biomarkers. Stenotrophomonas and Sphingobacterium are of particular interest given their positive association with epizootic dynamics in the wild, but negative association with Bd clearance in the laboratory (Supplementary Data 22). The high betweenness centrality of Sphingobacterium in the epizootic bacteria-metabolite network supports its importance in driving differences in microbiome function based on disease. Batrachochytrium was identified as the most common fungal taxa associated with metabolome phenotype and also had the greatest betweenness centrality in the wild epizootic population, indicative of Bd-driven metabolic perturbation, as previously shown in moribund frogs [72]. Links between multiple microbial taxa and single metabolite features may resemble functional redundancy within a population, whereby multiple taxa utilise/produce the same metabolite feature [73]. Alternatively, metabolite production may be contingent on interactions between microbial taxa, such as the anti-Bd metabolite tryptophol, which is produced in bacterial co-culture but not in monoculture [17]. Associations between microbial taxa and individual metabolites may also be important for microbiome community assembly, if the presence of one taxon is dependent on the production of a metabolite by another, for example through cross-feeding mechanisms [74]. Investigating microbe-metabolite interactions further will therefore be valuable in enhancing our understanding of the ecological processes governing the amphibian skin microbiome and its role in disease.

Conclusion
We applied 16S and ITS2 high-throughput amplicon sequencing, shotgun metagenomics and metabolomics to establish the relationship between amphibian skin microbiome taxonomic profile and community function. We demonstrate that bacterial and fungal taxonomic community composition strongly predicts skin metabolome profile, indicating a lack of functional redundancy. Further, we show that Bd drives changes in microbiome community structure, bacterial gene abundance and skin metabolome during laboratory infection and in a wild outbreak. Our integration of complex biological data and identification of shared multi-omics features in the laboratory and field provide mechanistic insight into the role of the microbiome in Bd infection outcome. These results lay the foundations for future studies examining microbiome-Bd interactions and will be valuable towards developing biomarkers as early warning beacons of epizootics in nature.