TLR4 regulates RORγt+ regulatory T-cell responses and susceptibility to colon inflammation through interaction with Akkermansia muciniphila

Well-balanced interactions between gut microbiota and the immune system are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD). Toll-like receptor 4 (TLR4) functions as a sensor mediating the crosstalk between the intestinal commensal microbiome and host immunity, but the influence of TLR4 on the shaping of intestinal microbiota and immune responses during colon inflammation remains poorly characterized. We investigated whether the different susceptibilities to colitis between wild-type (WT) and TLR4−/− mice were gut microbiota-dependent and aimed to identify the potential immunity modulation mechanism. We performed antibiotic depletion of the microbiota, cohousing experiments, and faecal microbiota transplantation (FMT) in WT and TLR4−/− mice to assess the influence of TLR4 on intestinal microbial ecology. 16S rRNA sequencing was performed to dissect microbial discrepancies, and dysbiosis-associated immune perturbation was investigated by flow cytometry. Akkermansia muciniphila (A. muciniphila)-mediated immune modulation was confirmed through the T-cell transfer colitis model and bone marrow chimaera construction. TLR4−/− mice experienced enhanced susceptibility to DSS-induced colitis. 16S rRNA sequencing showed notable discrepancy in the gut microbiota between WT and TLR4−/− mice. In particular, A. muciniphila contributed most to distinguishing the two groups. The T-cell transfer colitis model and bone marrow transplantation (BMT) consistently demonstrated that A. muciniphila ameliorated colitis by upregulating RORγt+ Treg cell-mediated immune responses. Mucosal biopsies from human manifested parallel outcomes with colon tissue from WT mice, as evidenced by the positive correlation between TLR4 expression and intestinal A. muciniphila colonization during homeostasis. Our results demonstrate a novel protective role of TLR4 against intestinal inflammation, wherein it can modulate A. muciniphila-associated immune responses. These findings provide a new perspective on host-commensal symbiosis, which may be beneficial for developing potential therapeutic strategies. CehHU1DpMuC43wfy6GXbFM Video abstract. Video abstract.


Introduction
Inflammatory bowel disease (IBD) encompasses two phenotypes, Crohn's disease (CD) and ulcerative colitis (UC), which are characterized by chronic relapsingremitting inflammatory disorder of the gastrointestinal tract [1,2]. Although the exact aetiology remains unclear, it has long been recognized that the pathogenesis of IBD consists of a combination of genetic susceptibility, environmental exposure, gut microbiota disturbances, and immune system dysfunctions [3][4][5]. Notably, microbial dysbiosis, defined as a decrease in gut microbiome diversity owing to a shift in the balance between commensal Graphical Abstract and potentially pathogenic microorganisms, contributes to the occurrence of IBD [6][7][8]. Emerging evidence indicates that excessively activated immune responses, especially toll-like receptor (TLR)-dependent immune dysfunctions mediated through perturbations in the intestinal microbiome, play key roles in the etiopathogenesis of IBD [9][10][11].
Toll-like receptor 4 (TLR4), an essential member of the pattern-recognition receptor (PRR) family, functions as a key sensor of intestinal microbiota alterations and specifically recognizes pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) in the intestine [12,13]. Increased expression of TLR4 is observed in epithelial and lamina propria cells of patients with IBD [14,15]. Previous studies have demonstrated that TLR4 activation results in the transcription of inflammatory and immunoregulatory genes, and subsequent downstream signalling pathway cascades participate in the progression of IBD [16]. A large amount of evidence supports a negative proinflammatory role of the TLR4 signalling pathway in IBD. Interestingly, genetic mutations and dysregulations of TLRs (including TLR4) are associated with a markedly enhanced predisposition and susceptibility to IBD in animal models, indicating that TLR4 is required for the intestinal response to epithelial injury and commensal microflora recognition [17,18]. TLR4 thus has dual roles in IBD; on the one hand, it can amplify inappropriate immune responses that ultimately cause chronic inflammation; on the other hand, it is necessary for maintaining tolerance and eliminating pathogenic microorganisms during steady-state conditions [19]. The impact of TLR4 on the aetiology of IBD is multidimensional and multifactorial, involving interactions among genetics, gut microbiota, and immune responses.
Prior studies have demonstrated that TLR4-deficient mice develop severe DSS-induced colitis, which is linked to impaired intestinal barrier function and changes in the inflammatory cytokine profile [18,20]. Although TLR4 plays a decisive role in maintaining immune tolerance and gut homeostasis, its role in shaping colonic bacterial ecology and microbiota-associated immunity has not been investigated in depth. In this study, we investigated the impact of TLR4 on the shaping of intestinal microbiota and host immunity during colon inflammation. Here, we show that intestinal microbiota dysbiosis caused by the loss of TLR4 gives rise to imbalanced immune responses and an enhanced predisposition to colitis in mice. The reduced abundance of Akkermansia muciniphila (A. muciniphila) and the decreased frequency of suppressive RORγt + Treg cells in TLR4 −/− mice contribute to the enhanced susceptibility to colon inflammation. These findings provide a new perspective on host-commensal symbiosis, which may be beneficial for the development of potential therapeutic strategies to alleviate IBD.

Results
Exacerbated colitis in TLR4 −/− mice depends on the gut microbiota TLR4 gene expression was upregulated in the intestinal epithelia of patients with UC, indicating that TLR4 might be a participant in UC development (Fig. 1a). We observed that TLR4 −/− mice developed severe colitis, as evidenced by increased weight loss (Fig. 1b), decreased survival rate (Fig. 1c), a higher DAI score (Fig. S1a), and a shortened colon length (Fig. S1 b and c). Pronounced colon inflammation in TLR4 −/− mice was also obvious based on haematoxylin and eosin (H&E) staining of the histology score ( Fig. S1 d and e) and endoscopic evaluations of the colonoscopy score ( Fig. S1 f and g).
Principal coordinate analysis (PCoA) using the Bray-Curtis metric distance and weighted UniFrac distance algorithms was performed to evaluate the beta diversity. An apparent clustering separation between amplicon sequence variants (ASVs) revealed the difference in community structures between WT and TLR4 −/− mice ( Fig. 2b and d). Comparison of within-and betweengroup dissimilarity analyses revealed that the microbiome difference between WT and TLR4 −/− mice was greater than the difference within each genotype (Fig. 2c, calculated from Fig. 2b; Fig. 2e, calculated from Fig. 2d). We assessed the general landscape of the gut microbiota in all available samples to further investigate the potential compositional differences at various taxonomic levels ( Fig. S2 a-c). The intestinal flora composition between different genotypes can be segregated by a comparison heatmap based on the ASV abundance at both the family (Fig. 2f ) and genus levels (Fig. 2g). Lactobacillaceae (the family and genus Lactobacillus) and Akkermansiaceae Fig. 1 Gut microbiota distinguishes the colitis severity in WT mice from that in TLR4 −/− mice. a TLR4 expression is upregulated in tissue samples from UC patients by NCBI GEO database. (GSE11223: healthy sample, n = 73, UC patients, n = 128; GSE87466: healthy sample, n = 21, UC patients, n = 87; GSE126124: healthy sample, n = 47, UC patients, n = 36). b Body weight change. c Survival. d Body weight change. e Survival. f Disease activity index (DAI) score. g Representative pictures of colon gross appearance. h Colon length. i Representative microscopic pictures of H&E staining (40× and 200× magnification). j Histology score. k Representative colonoscopy images. (l) Colonoscopy score. (b-l) n = 10 mice per group, mean values ± SEM are presented, and p-values were calculated using unpaired T-test, *p < 0.05, **p < 0.01, ***p < 0.001. Data are pooled from three independent experiments with n = 10 mice per group (the family and genus Akkermansia) displayed a significant enrichment in WT mice, while Rikenellaceae (the family and genus Rikenella) showed a relatively high abundance in TLR4 −/− mice. Collectively, these results demonstrated that the intestinal microbiome in WT mice is obviously differed from TLR4 −/− mice.
A predisposing microbiota in TLR4 −/− mice is responsible for the enhanced susceptibility to colitis To investigate whether the gut microbiota in TLR4 −/− mice was responsible for the enhanced susceptibility to colitis, we performed FMT experiments in which pseudosterile WT or TLR4 −/− recipient mice were reconstructed with the microbiome from WT or TLR4 −/− donor mice ( Subsequently, spontaneous microbiota transfer studies between different genotypes were performed through cohousing experiments. Age-and sex-matched WT and TLR4 −/− littermates were either housed singly ('SiHo mice') or cohoused ('CoHo mice') for 6 weeks prior to DSS administration (Fig. 3a). CoHo TLR4 −/− mice and their WT cage mates (CoHo WT mice) exhibited a similar phenotype following microbiota exchange through coprophagia, as demonstrated by the corresponding body weight loss (Fig. 3b), survival rate (Fig. 3c), DAI score (Fig. 3d), colon length ( Fig. 3e and f ), and histology score ( Fig. 3g and h). Consistently, the microbiome compositional structure of CoHo TLR4 −/− mice was comparable to that of CoHo WT mice, as evidenced by the overlapping coordinates and locations based on PCoA of beta diversity (Fig. S4 d-f, Fig. S6 a-c). Taken together, the predisposing microbiota in TLR4 −/− mice was responsible for the enhanced susceptibility to colon inflammation.

Colonic suppressive RORγt + Treg cells mitigate colitis in a gut microbiota-dependent manner
To investigate the imbalance in immune responses between WT and TLR4 −/− mice, the immune status of the intestinal microenvironment was evaluated. The frequency of both subpopulations of colon-resident macrophages [(R1 fraction, CD11b + CD11c low F4/80 + CD103 − macrophages) and (R2 fraction, CD11b + CD11c + F4/80 + CD103 − macrophages)] displayed no significant difference between the two genotypes ( Fig. S7 a-c). In addition, the population of CD11b low CD11c + F4/80 − CD103 + (R3 fraction) colonic dendritic cells (DCs) showed a comparable tendency between WT and TLR4 −/− mice ( . Increasing evidence indicates that RORγt + Treg cells represent a stable regulatory T-cell effector lineage, with reinforced anti-inflammatory and immunesuppressing effects during colitis [21,22]. Given the immunological crosstalk between the gut and other tissue compartments, we investigated whether the observed differences in RORγt + Treg cell responses in the gut could be detected more systemically. However, RORγt + Treg cells were not found in peripheral lymphoid organs (such as the spleen) ( Fig. 4a and c). Additionally, we confirmed the RORγt and Foxp3 expression in colon tissue using immunofluorescence double staining, and coexpression of RORγt and Foxp3 in T-cell subsets was significantly higher in WT than in TLR4 −/− mice ( Fig. 4d and e). Correlation analysis results showed that the ratio of suppressive RORγt + Treg cells in the colonic LP was negatively associated with the colitis phenotype (colon length in Fig. S10a, DAI score in Fig. S10b, histology score in Fig. S10c, colonoscopy score in Fig. S10d).
To illustrate whether the colonic RORγt + Treg cell response was gut microbiota-dependent, the particular T-cell subsets were also phenotyped  These results demonstrated that the frequency of colonic RORγt + Treg cells was negatively correlated with colitis severity, and that suppressive RORγt + Treg cells mitigated colitis in a gut microbiota-dependent manner.

Akkermansia muciniphila is negatively related to the colitis phenotype in a murine model and in UC patient samples
Correlation analysis between differential flora and phenotypic indicators suggested that the Akkermansia muciniphila (A. muciniphila) abundance was negatively correlated with the DAI (WT, To determine the correlation between A. muciniphila and chronic colon inflammation, 16S rRNA gene highthroughput sequencing was conducted in faecal bacterial DNA isolated from paired UC patients and healthy controls. PCoA revealed an apparent clustering separation between UC patients and healthy participants in terms of the microbiome structures (Fig. S13a). Remarkably altered bacterial strains were identified by a comparison heat map of UC patients with healthy participants, and A. muciniphila abundance was significantly downregulated in patients with UC (Fig. S13b). LEfSe analysis also indicated that A. muciniphila was the predominant biomarker in healthy participants compared with UC patients (Fig. S13c). Additionally, we downloaded the published raw 16S rRNA gene sequencing data [23] Fig. S17 a and b) of UC patients revealed a decreased abundance of A. muciniphila compared with the healthy population. Taken together, the A. muciniphila abundance was negatively related to colitis risk, not only in murine models but also in UC patient samples.

Akkermansia muciniphila supplementation suppresses colon inflammation and increases the frequency of colonic RORγt + Treg cells
We inoculated WT and TLR4 −/− mice with A. muciniphila, and colonization was identified by 16S rRNA sequencing (Fig. S18). Mice that were administered A. muciniphila ([WT Akk] or [TLR4 −/− Akk]) and control mice that received the brain-heart infusion (BHI) vehicle ([WT BHI] or [TLR4 −/− BHI]) had similar body weights before DSS treatment (Fig. 6a). Strikingly, [WT Akk] and [TLR4 −/− Akk] mice exhibited decreased colon inflammation, including reduced body weight loss (Fig. 6a), decreased mortality (Fig. 6b), lower DAI scores (Fig. 6c), lower colon histopathology scores ( Fig. 6f and g), and greater colon lengths ( Fig. 6d and  Previous studies have indicated that A. muciniphila induces gut microbiota remodelling and activates anti-inflammatory Treg cell responses [23,24]. In our study, colonic tissue Foxp3 and RORγt expression were both positively correlated with A. muciniphila colonization (Fig. S19 a and b), suggesting a potential induction of Foxp3 and RORγt expression by A. muciniphila. Consistent with the phenotype, A. muciniphila administration significantly increased colonic RORγt + Treg cell responses compared with the BHI vehicle controls ( Fig. 6h and i), and A. muciniphila supplementation did not change the frequency of RORγt + Treg cells in the mouse spleen ( Fig. 6h and j). Colonic innate immune responses were also phenotyped, and A. muciniphila administration did not significantly transform macrophage and DC immune responses (Fig. S20 a-d).
To demonstrate the specific immune regulation of RORγt + Treg cells by A. muciniphila, a T-cell transfer model, which is dependent on both T cells and microbiota, was conducted in our study. At 3 weeks prior to the T-cell transfer treatment, we inoculated Rag1-deficient (Rag1 −/− ) mice with A. muciniphila or BHI medium via oral gavage for 3 weeks (Fig. 7a). Mice colonized with A. muciniphila or BHI medium had comparable initial body weights before T-cell transfer (Fig. 7b). Colon inflammation evaluation was conducted 4 weeks following T-cell transfer treatment. As measured by body weight loss (Fig. 7b), colon length ( Fig. 7c and d), and histology score ( Fig. 7e and f ), colon inflammation was more severe in mice colonized with BHI (Rag1 −/− + BHI) than in mice colonized with A. muciniphila (Rag1 −/− + Akk). In particular, A. muciniphila supplementation notably elevated the ratio of colonic RORrt + Treg cells in Rag1 −/− + Akk mice (Fig. 7g-j).
To investigate immune regulation through intestinal epithelial cell or bone marrow-derived cell TLR4

The interaction between TLR4 and Amuc-1100 mediated colonization of A. muciniphila
Considering the decreased abundance of A. muciniphila in TLR4 −/− mice, we proposed that TLR4 might affect the intestinal colonization of A. muciniphila. Colon tissue from both healthy participants and WT mice indicated that TLR4 expression was positively correlated with A. muciniphila colonization during gut homeostasis (Fig. S23 a and b). FISH staining of colonic sections from WT mice revealed increased hybridization of the A. muciniphila probe MUC1437 [25,26] compared with those from TLR4 −/− mice (Fig. S23c). Based on the genomic and proteomic analysis of A. muciniphila, the pili-like external membrane protein Amuc-1100 might participate in the colonization of A. muciniphila [27,28]. Subsequently, we downloaded the molecular model of TLR4 (Fig. S24a) and also constructed the 3D structure model of protein Amuc-1100 (https:// zhang lab. ccmb. med. umich. edu/I-TASSER/) on the basis of its amino acid sequence (Fig. S24b). The configurations of TLR4 and Amuc-1100 were simulated through Z-DOCK, and the 10 top-ranked possible complex scenarios were  (Figs. S25-27). According to the energy-based scoring system, the complex 2 manifested the most likely complex scenario one (Fig. S24 c and d). Collectively, our results indicated that the interaction between TLR4 and Amuc-1100 might mediate the intestinal colonization of A. muciniphila.

Discussion
Well-balanced interactions between gut microbiota and host immune systems are essential to maintain intestinal homeostasis. As a key member of the PRR family, TLR4 mediates the crosstalk and interplay between the intestinal commensal microbiome and host immune systems. In this study, we investigated the protective role of TLR4 in the shaping of colonic bacterial compositions and microbiota-associated immunity against colon inflammation. Genetic deletion of TLR4 has drastic consequences on the structure and composition of the intestinal microbial communities, leading to a shift towards a proinflammatory configuration that drives enhanced susceptibility and vulnerability to colitis. The decreased abundance of the predominant bacterium A. muciniphila and the reduced proportion of suppressive RORγt + Treg cells in TLR4 −/− mice contribute to the enhanced susceptibility to colon inflammation. Therefore, our results demonstrate that TLR4 is an indispensable regulator and keeper of gut homeostasis.
The gastrointestinal tract is populated by trillions of diverse and complex microorganisms that function as key players in energy metabolism and host susceptibility to multiple intestinal conditions and diseases [29,30]. Previous studies have indicated that TLR4 deficiency renders mice susceptible to DSS-induced colitis, which is associated with impaired intestinal barrier function and changes in the inflammatory cytokine profile [18,20]. Unlike conventionally raised TLR4 −/− mice, which are more susceptible to colitis than WT mice, ABX(TLR4 −/− ), mice exhibited indistinguishable colon inflammation following broad-spectrum antibiotic treatment for gut microbiota deprivation in our study when compared with ABX(WT) mice. Our results indicated that resident intestinal bacteria were required for the enhanced susceptibility of TLR4 −/− mice to colitis. Gut microbiota dysbiosis, which is usually characterized by the loss of beneficial commensal microflora, expansion of pathogenic bacteria, and reduced overall biodiversity of the microbial ecosystem, is associated with the pathogenesis of IBD [31,32]. These compositional abnormalities and structural alterations could be both the cause and consequence of IBD, thus inducing a vicious cycle of persistent inflammatory responses. Previous studies have shown that a deficiency in NOD2 or NLRP6 results in a colitogenic microbiota that can exacerbate DSS-induced colitis, and the aggravated colitis phenotype exhibited by NOD2 −/− or NLRP6 −/− mice could be transferred to WT mice by cohousing [33,34]. Our cohousing and bacteriatransfer experiments collectively demonstrate that dysbiosis and exacerbated colitis caused by the loss of TLR4 can be partially reversed by transferring gut microbiota from WT mice, further supporting our conclusion that TLR4 is functionally important for the maintenance of intestinal homeostasis and that its deficiency shapes a transmissible, disease-predisposing intestinal microflora.
IBD is characterized by unresolved inflammation in the intestinal tract, which is controlled by a complex interplay of innate and adaptive immune mechanisms [35,36]. Multiple innate and adaptive immune cells and cytokines in time and space orchestrate the development, recurrence, and exacerbation of the imbalanced inflammatory process in IBD [37]. The correlation of the gut microbiome with host immunity involves a bidirectional relationship between microbes and the host innate and adaptive immune systems [4]. The presence of large numbers of symbionts poses an enormous challenge to the host immune system because it must activate robust immune responses to eliminate invading pathogens while maintaining self-tolerance to avoid autoimmune responses against symbiotic flora [38]. Excessive activation of immune systems triggered by bacterial disturbance is recognized as the underlying mechanism of chronic intestinal inflammatory responses in IBD [32]. Accumulating evidence indicates that RORγt + Treg cells represent a stable regulatory T-cell effector lineage with reinforced anti-inflammatory and immune-suppressing effects during intestinal-specific inflammation [39]. Compared with the gut microbiota from healthy donors, transfer of the IBD donor microbiota into germ-free mice resulted in exacerbated colitis accompanied by a significantly decreased proportion of colonic RORγt + Treg cells, suggesting a potential immuneassociated mechanism for microbial contribution to IBD pathogenesis [38,40]. In this study, aggravated colon inflammation in TLR4 −/− mice was accompanied by a significantly reduced proportion of RORγt + Treg cells, while mitigated colitis in WT mice was accompanied by a relatively higher frequency of suppressive RORγt + Treg cells. A large body of evidence indicates that intestinal RORγt + Treg cells are highly context-dependent and have functions in promoting host immunity, particularly constraining immunoinflammatory responses under inflammatory conditions [41,42]. Consistent with our phenotype, CoHo TLR4 −/− and FM(WT)→TLR4 −/− recipient mice displayed ameliorated colitis and elevated colonic RORγt + Treg cells, indicating that colonic suppressive RORγt + Treg cells mitigated colitis in a gut microbiota-dependent manner.
Among the next-generation beneficial microbes that have been identified, A. muciniphila is strongly positioned in the forefront of candidates. Belonging to the Verrucomicrobia phylum, the gram-negative anaerobic commensal A. muciniphila can degrade mucin and is an abundant member of the human intestinal microbiota [43][44][45]. A. muciniphila is inversely associated with multiple metabolic disorders and chronic inflammation, and colonization with A. muciniphila has been reported to have protective effects against high-fat diet (HFD)induced obesity, facilitate mucosal wound healing, and elevated antitumour responses during anti-PD-1 immunotherapy [46][47][48]. Consistently, we observed a marked decrease in the enrichment of A. muciniphila in stool samples and intestinal tissue biopsies of patients with UC, and A. muciniphila abundance was negatively correlated with colitis risk in our animal models. Evidence indicates that A. muciniphila administration ameliorates DSS-induced colitis in mice either via microbehost interactions, which protect gut barrier function and reduce the levels of inflammatory cytokines, or by improving the microbial community [43]. As expected, A. muciniphila supplementation markedly ameliorated colitis not only in the WT genotype but also in the TLR4-deficient genotype in our study. In particular, colonic tissue Foxp3 and RORγt expression were both positively correlated with A. muciniphila colonization, and A. muciniphila administration significantly increased colonic RORγt + Treg cell responses compared with the BHI controls. Studies have shown that A. muciniphila induces intestinal adaptive immune responses during homeostasis, and that A. muciniphilaspecific contextual signals influence T-cell responses to the microbiota and modulate host immune function [49]. Oral administration of A. muciniphila induces gut microbiota remodelling in nonobese diabetic (NOD) mice, which is associated with the promotion of Foxp3 + regulatory T cells in islets and interleukin 10 and transforming growth factor β in pancreatic lymph nodes [24]. HFD-fed mice administered A. muciniphila show improved glucose tolerance and an increased number of adipose tissue-resident CD4 + Foxp3 regulatory T cells [50]. Therefore, we hypothesized that A. muciniphila could specifically immunoregulate colonic RORγt + Treg cells in our study. A recent study highlighted that mice colonized with IBD microbiota experienced more severe disease and a decreased ratio of RORγt + Treg cells than those colonized with healthy donor microbiota based on a T-cell transfer model of colitis [38]. In our study, susceptible Rag1 −/− mice were inoculated with A. muciniphila or BHI medium prior to T-cell transfer treatment, and Rag1 −/− + Akk mice experienced a mitigated colitis phenotype and an increased percentage of colonic RORrt + Treg cells compared with Rag1 −/− + BHI mice, indicating the potential immune induction of colonic RORrt + Treg cells by A. muciniphila in a specific context.
Although the beneficial value of A. muciniphila as a potential probiotic has been widely recognized, adverse effect may also exist. A prior study demonstrated that the presence of A. muciniphila exacerbated the severity of colon inflammation caused by Salmonella typhimurium (S. typhimurium) infection in mice colonized with a simplified human gut microbiota (SIHUMI) [51]. The presence of A. muciniphila alone in mice is not pathogenic; when both A. muciniphila and S. typhimurium were present in SIHUMI mice simultaneously, colonic inflammation was exacerbated because of the decreased level of IL-18 and macrophage dysfunction. Seregin et al. reported that A. muciniphila was sufficient to induce colon inflammation in an IL-10 knockout (IL-10 −/− ) model of colitis [52]. This conflicting conclusion may be explained by various factors, such as the different mouse models used. This phenotype is highly dependent on the genetic background of the specific IL-10 knockout model. Evidence indicated that different commensal bacteria, such as Escherichia coli and Enterococcus faecalis, could induce immune-mediated inflammation in IL-10 −/− mice, but neither bacterial strain caused colon inflammation in wild-type mice [53]. Therefore, the colitogenicity of A. muciniphila is context dependent. When gut homeostasis is disrupted, beneficial microbes may switch to potentially virulent species, which may exert harmful effects on the host.
Gene-microbiota interactions contribute to the pathogenesis of IBD, and mutations in genetic pathways linked to IBD result in an inability to sense and/or respond to beneficial microbes [54,55]. Previous studies demonstrated that NLRP12 deficiency in mice promotes a dysbiotic microbiome, and that NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and boosting the growth of the protective gut commensal strain (of the family Lachnospiraceae) [56]. Host gene caspase recruitment domain family member 9 (CARD9) affects the composition and function of the gut microbiota, altering the production of microbial metabolites and intestinal inflammation [57]. Evidence indicates that arginase 1 (Arg1) expression impedes the resolution of intestinal inflammation by altering the faecal microbiome and the metabolome [58]. Moreover, unlike pathogens with TLR ligands triggering inflammation, some commensal bacteria exploit the TLR pathway to actively suppress immune reactions [59]. As observed in the interplay between A. muciniphila and suppressive RORγt + Treg cells in our study, TLR4 is required for shaping the colonic ecology and maintaining intestinal homeostasis.
Correlation analysis results indicate that TLR4 expression is positively related to A. muciniphila colonization in both human and mouse samples. Combined with our FISH staining results showing that intestinal A. muciniphila colonization manifested significant accumulation during homeostasis in WT mouse samples, we hypothesized that TLR4 could exert an influence on the intestinal colonization of A. muciniphila. Genomic and proteomic analysis of A. muciniphila indicated that external membrane protein Amuc-1100 might participate in the colonization process [27,28], and our docking results also demonstrated the interaction between TLR4 and Amuc-1100. Further in-depth mechanisms will be explored in future research.

Conclusions
In conclusion, our study identified an unexpected role for TLR4 in regulating intestinal microbiota compositions and susceptibility to colon inflammation through shaping the colonization of A. muciniphila. In particular, A. muciniphila-based mechanisms play a fundamental role in driving the divergent induction of suppressive RORγt + Treg cells in the gut-specific microenvironment. Therefore, based on the interplay and crosstalk between gut microbiota and immune responses, our results may offer alternative avenues for therapeutic intervention in IBD.

Patients
All participants were recruited via the Department of Gastroenterology, The Second Affiliated Hospital of Third Military Medical University. Participants were excluded if they had used antibiotics, sulfasalazine, probiotics, or prebiotics in the month preceding faecal sampling, as this could influence the intestinal microbiota composition and structure. We collected faecal samples from 29 UC patients and 35 age-and sex-matched healthy controls. Both patients and healthy subjects were Chongqing habitants and consumed an eastern diet. Intestinal biopsy specimens were collected from 62 healthy participants who underwent colonoscopy for dyspeptic symptoms at the Digestive Endoscopy Center of The Second Affiliated Hospital of Third Military Medical University. The research group strictly followed the guidelines of the Declaration of Helsinki and the principle of biomedical research involving human norms of international ethics established by the WHO and CIOMS. The study was approved by the Medical Ethics Committee of The Second Affiliated Hospital of Third Military Medical University (No. AMWUEC2020582). Written informed consent was provided by all participants before collection of the samples.

Mice
All animal experimental protocols were performed following the guidelines of the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Laboratory Animals Welfare and Ethics Committee of Third Military Medical University (No. AMWUEC2020582). TLR4-knockout (TLR4 −/− ) mice on the C57BL/6J background were obtained from GemPharmatech (Co., Ltd., Jiangsu, China). Both TLR4 −/− mice and wild-type (WT) C57BL/6J mice were originally generated from the same breeders and house raised at the Animal Center of Third Military Medical University Second Affiliated Hospital for at least ten generations. Ageand sex-matched WT and TLR4 −/− littermates were employed in our study. All animals were maintained in the same room and generally in the same facilities (such as racks and litters). They were maintained by the same personnel, who changed their gloves and used sterile utensils to prevent transfer of microbes between cages. Throughout the acclimatization and study periods, all animals were maintained on a 12-h light-dark cycle (21 ± 2 °C with a relatively constant humidity of 45 ± 10%) under specific pathogen-free (SPF) conditions and had access to food and water ad libitum. All mice were fed irradiated food and maintained in autoclaved cages.

DSS-induced colitis model
To induce acute experimental colitis, mice were administered 1.5-2.5% (w/v) dextran sodium sulphate (DSS, molecular weight, 36-50 kDa; MP Biomedicals, UK) in their drinking water ad libitum for 7 days. To assess experimental colitis and repair, a recovery model was implemented; specifically, mice were administered 2.5% (w/v) DSS in their drinking water ad libitum for 7 days followed by 7 days of normal water. In all colitis models, mice were checked daily for morbidity, and body weight was recorded. Each mouse was scored for pathological features, including stool consistency, the presence of blood in the stool, and body weight loss. Individual scores were combined to generate the disease activity index (DAI), which was calculated daily for each mouse. The maximum score was 12 based on assigning a 0-4 scoring system for the following parameters: weight loss (0 points = 0% weight loss from baseline; 1 point = 1-5% weight loss; 2 points = 5-10% weight loss; 3 points = 10-20% weight loss; and 4 points = more than 20% weight loss); rectal bleeding (0 points = negative; 2 points = positive hemoccult test; and 4 points = visible bleeding); and stool consistency (0 points = normal faeces; 1 point = loose stool; 2 points = watery diarrhoea; 3 points = slimy diarrhoea, little blood; and 4 points = severe watery diarrhoea with blood).

T-cell transfer colitis model
T-cell transfer colitis experiments were performed as previously described [38]. Briefly, CD4 + T cells were isolated from the spleen and subcutaneous lymph nodes of 6-to 8-week-old SPF C57BL/6J mice. CD4 + T cells were enriched using negative magnetic selection (Miltenyi Biotec) following tissue dissociation and red blood cell lysis. Isolated cells were stained with CD4, CD25, and CD45RB, ensuring that CD45RB high CD4 + T cells accounted for ~50% of the fraction. Immunodeficient Rag1-deficient (Rag1 −/− ) mice (8-10 weeks old, male) on a C57BL/6 background were employed as recipients in our transfer model. Each recipient received an intravenous injection of a minimum of 4 × 10 5 purified cells (200 μL/injections). Colitis evaluation was conducted 4 weeks following T-cell transfer.

Histopathology
The colons were emptied of faecal contents, opened longitudinally along the mesenteric border, formed a Swiss roll from the proximal to the distal end, and then placed in 10% neutral buffered formalin for 24 h. The Swiss rolls were transferred to 70% ethanol and then processed into paraffin-embedded blocks to generate 5-μm-thick sections for haematoxylin and eosin (H&E) staining. The sections were evaluated by an experienced pathologist in a blinded manner, and histological scores were assessed according to the following parameters: inflammation, epithelial defects, crypt atrophy, dysplasia/neoplasia, and the area affected by dysplasia. Each parameter generated a separate score with a value between 0 and 4 based on the colon inflammation severity and extent [60,61].

Colonoscopy evaluation
Colonoscopy was performed on experimental mice using a high-resolution mouse video endoscopic system (KARL STORZ, Tuttlingen, Germany). The severity of colitis was scored in a blinded manner using MEICS (murine endoscopic index of colitis severity) based on the following 5 parameters: (a) transparency of the colon, (b) changes in the vascular pattern, (c) fibrin visible, (d) granularity of the mucosal surface, and (e) stool consistency [62,63]. Each parameter was scored with a value between 0 and 3. The cumulative score ranged from 0 (no signs of inflammation) to 15 (signs of very severe inflammation).

Bone marrow transplantation (BMT)
Recipient mice (8-10 weeks old, male, WT or TLR4 −/− mice) received lethal irradiation with a total dose of 11 Gy in two 5.5-Gy fractions separated by 4-5-h intervals. To minimize heterogeneity, bone marrow cells were normally obtained from the long bones of donor mice (8-10 weeks old, male, WT or TLR4 −/− mice) with recipient mice of similar age. Harvesting of bone marrow cells for transplant must be done rapidly after death without intervention via chemical means that could complicate the functional status of stem cells. Each recipient mouse typically received 2 × 10 7 bone marrow cells for engraftment. Peripheral blood was assessed by flow cytometry for the percentage of CD45.1 (donor) and CD45.2 (recipient) leukocytes using standard techniques 4 weeks following transplantation [64]. We

Immunofluorescence staining
For immunofluorescence staining, colonic segments were embedded in Cryomatrix and frozen on dry ice. Cryosections (10 μm) were fixed for 5 min in 4% paraformaldehyde in PBS, washed in PBS-0.05% Tween 20 (PBT), incubated for 30 min at room temperature in PBT-5% normal goat serum (saturation buffer), and then incubated overnight at 4 °C with primary antibodies diluted in saturation buffer. Sections were counterstained with 4,6-diamidino-2-phenylindole (DAPI) for nuclear staining. Slides were dried and mounted using ProLong Antifade mounting medium (Invitrogen, Molecular Probes, Eugene Oregon). Slides were visualized using a Leica TCS SP5 confocal microscope. The following antibodies were used: AF647-anti-Foxp3 and PE-anti-RORγt. The number of positive cells per field of view under ×800 magnification was counted, and data were collected from ten randomly selected fields.

Cultivation of A. muciniphila and mouse colonization with A. muciniphila
A. muciniphila MucT (ATCC BAA835) was cultured under strictly anaerobic conditions at 37 °C in brainheart infusion (BHI) medium as described previously [24,65], and exponentially growing cultures were washed with PBS and immediately frozen in PBS containing 25% glycerol to a final concentration of 1 × 10 10 cells per mL [66]. Prior to administration, a frozen pellet of A. muciniphila was thawed and resuspended in anaerobic PBS to a concentration of 1.5 × 10 9 per mL. Mice were treated by oral gavage with a bacterial A. muciniphila suspension in BHI twice a week for 3 weeks. BHI broth was used as a vehicle control. After A. muciniphila or BHI supplementation, the mice were administered DSS 1 week after the final gavage.

Statistical analysis
Statistical analysis was performed with GraphPad Prism 7.0 software (GraphPad Software Inc., San Diego, USA). Significance between two groups was determined using the unpaired, two-tailed Student's t-test, and significance between multiple groups was determined by two-way analysis of variance (ANOVA) with Fisher's LSD test. The results are shown as the mean ± SEM; statistical significance is indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001, and NS means no significance.
Additional file 1: Figure S1. TLR4 -/mice develop severe DSS-induced colitis. Figure S2. Loss of TLR4 significantly alters gut microbiota taxonomic composition. Figure S3. FMT alleviates colon inflammation in TLR4 -/mice. Figure S4. Gut microbiota PCoA profile of Co-housing and FMT experiments. Figure S5. Gut microbiota taxonomic composition in FMT experiments. Figure S6. Gut microbiota taxonomic composition in Co-housing experiments. Figure S7. The intestinal innate immune responses evaluation between WT and TLR4 -/mice. Figure S8. The intestinal adaptive immune responses evaluation between WT and TLR4 -/mice. Figure S9. The cytokines profile of Treg and Th17 cells between WT and TLR4 -/mice. Figure S10. Correlation analysis between RORγt + Treg cells and clinical parameters. Figure S11. Correlation analysis between differential flora and phenotypic indicators. Figure S12. Gut microbiota landscope of Co-housing and FMT experiments. Figure S13. The relative abundance of A. muciniphila is decreased in stool samples in patients with UC. Figure S14. The microbiome of UC patients are different from healthy participants. Figure S15. The microbiome of UC patients are different from healthy participants. Figure S16. The microbiome of UC patients are different from healthy participants. Figure S17. The microbiome of UC patients are different from healthy participants. Figure S18. A. muciniphila abundance discrepancy following single bacteria supplementation. Figure S19. Correlation analysis between transcription factor expression and A. muciniphila colonization. Figure S20. The intestinal innate immune responses evaluation between WT and TLR4 -/mice after A. muciniphila supplementation. Figure S21. Intestinal epithelial-derived TLR4 pathway participating in intestinal immune activation against colitis. Figure S22. A. muciniphila abundance discrepancy in BMT experiment following bacteria supplementation. Figure S23. TLR4 affects the intestinal colonization of A. muciniphila during homeostasis. Figure S24. The Interaction between TLR4 and Amuc-1100 mediated the intestinal colonization of A. muciniphila. Figure S25-