Fig. 6

DCA facilitates TNBS-induced colonic inflammation. a–h Littermate WT mice were fed with drinking water or 0.2% DCA in drinking water for 4 weeks. Then, these mice were injected with or without TNBS via the rectal route. b The level of abdominal L-012 chemiluminescent signals in mice of indicated groups assessed on day 4 after TNBS treatment. c Survival rates of mice (n = 10) were monitored daily after TNBS treatment. d Body weight changes of mice (n = 5–6 per group) were monitored daily after TNBS administration. e Representative images of mouse colon in Control, DCA, TNBS, and TNBS+DCA groups. f The mice were sacrificed on day 4 after TNBS treatment, and their colon lengths were measured. g, h Colon sections were examined histologically. g Histology scores for colonic inflammation were measured. h Representative images of the H&E-stained colon sections of indicated groups (scale bars 100 μm). c, d, f, and g Data represent means ± SEM; *P < 0.05, **P < 0.01; by unpaired Student’s t test. The data shown are representative of three independent experiments