Fig. 2From: Maternal Western diet mediates susceptibility of offspring to Crohn’s-like colitis by deoxycholate generationAltered gut microbiota associated with MWD exacerbates TNBS-induced colitis. a–g Littermate WT mice were administered antibiotics for 3 days to remove gut microbiota, and then colonized with gut microbiota from W-N and N-N groups for 5 days. After that, mice were rectally injected with TNBS to induce colitis. b Survival rates of mice (n = 10) were monitored daily after TNBS administration. c Body weight changes of mice (n = 8) were monitored daily after TNBS administration. d Representative images of mouse colon in the N-N FMT and W-N FMT groups treated with TNBS. e The mice were sacrificed on day 4, and colon lengths were measured. f and g Colon sections were examined histologically. f Histology scores for colonic inflammation were measured. g Representative images of the H&E-stained colon sections of indicated groups (scale bars 100 μm). h Relative mRNA levels of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, and NOS2 were determined by real-time PCR and normalized to 18s. b, c, e, f, and h Data represent means ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001; by unpaired Student’s t test. The data shown are representative of three independent experimentsBack to article page