Fig. 2

M. rileyi infection activates the expression of three AMP genes at 48 hpi. A Unique and common proteins between the plasma collected from PBS (PPB)-injected and blastospore (PBL)-injected groups identified by LC–MS/MS analysis. The immune-related proteins unique to PBL are shown. The red and blue arrowheads represent the upregulated and downregulated expression, respectively. B Hierarchical clustering analysis of differentially expressed proteins (DEPs) shared in both PPB and PBL groups. All immune-related DEPs are indicated and AMPs are in red. C-E The RT-qPCR analysis showed that the expression of gloverin (C), cecropin 3 (D), and lebocin (E) was induced in the fat bodies from the larvae at 24 and 48 hpi of M. rileyi blastospores. The statistical differences were analyzed using Student’s t test (*p < 0.05, **p < 0.01, and ***p < 0.001)