Fig. 10

M. rileyi hyphal bodies or gut-derived bacteria with varied loads consume host amino acids from the hemolymph after infection. A Representative photographs indicate the hyphal bodies in the hemolymph of H. armigera larvae at 12, 24, 36, 48, and 60 hpi of M. rileyi blastospores. The hemolymph collected at 48 or 60 hpi was diluted (1:10) and photographed. The arrows indicate the hyphal bodies, whereas arrowheads represent the hemocytes. Scale bar = 50 μm. B The number of hyphal bodies in the hemolymph at 12, 24, 36, 48, and 60 hpi was determined. C The amino acid level in the hemolymph was measured at 12, 48, and 60 hpi of blastospores or PBS (control). D-F The varied load of A. johnsonii (D), S. rhizophila (E), or E. ludwigii (F) in the hemolymph from the larvae at 12, 24, 36, 48, and 60 hpi. The qPCR analysis was performed using specific primers of bacterial 16S rRNA gene and genomic DNA in the hemolymph as templates. G The amino acid level in the hemolymph was measured at 60 hpi of bacteria or PBS (control). The bacteria such as A. johnsonii, S. rhizophila, or E. ludwigii were injected individually. The data were analyzed by using Student’s t test (*p < 0.05) or one-way ANOVA followed by Tukey’s multiple comparison test. The different characters above the bars represent significant differences