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Fig. 1 | Microbiome

Fig. 1

From: Sialic acid exacerbates gut dysbiosis-associated mastitis through the microbiota-gut-mammary axis by fueling gut microbiota disruption

Fig. 1

Experimental design Cows were treated with a standard or high-grain diet for 8 weeks (A) and ruminal samples were collected for metabolomic analysis (B). C Untargeted metabolomics was performed by LC–MS and metabolites that were significantly different between healthy and SARA cows were identified. D The biological functions of sialic acid were validated in mice. E The underlying mechanism of potential metabolites on mastitis pathogenesis was investigated by fecal microbiota transplantation, precision editing of gut microbiota, in vitro experiments and probiotic treatment. F Ruminal microbiota transplantation and pharmacological sialidase inhibition were performed to confirm the role of sialic acid. LC–MS, liquid chromatography-mass spectrometry; Neu5Ac, N-acetylneuraminic acid; SARA, subacute ruminal acidosis; SCC, somatic cell count; SPF, specific pathogen free; SRMT, SARA ruminal microbiota transplantation

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Microbiome

ISSN: 2049-2618

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