Microbiome

Table 4 Antibiotic disk-diffusion susceptibility test results

From: Characterisation of the koala (Phascolarctos cinereus) pouch microbiota in a captive population reveals a dysbiotic compositional profile associated with neonatal mortality

		Antibiotic
		AMP25	TE10^a	CIP2.5	CN10^a	CAZ10	AZM15^a	C30	AMC15
ATCC1388	AR (mm)	0	4	14	9	9.5	4	10	6
ATCC1388	Profile	Resistant	Equivocal	Susceptible	Susceptible	Susceptible	Equivocal^b	Susceptible	Equivocal^b
ATCC33028	AR (mm)	0	0	12	2.5	9	7	2	4
ATCC33028	Profile	Resistant	Resistant	Susceptible	Resistant	Susceptible	Susceptible	Resistant	Resistant
CSK1-PG	AR (mm)	0	0	12	9	9	5	0	3
CSK1-PG	Profile	Resistant	Resistant	Susceptible	Susceptible	Susceptible	Susceptible	Resistant	Resistant
CSK2-PG	AR (mm)	0	2	14	9	12	6	3	4
CSK2-PG	Profile	Resistant	Resistant	Susceptible	Susceptible	Susceptible	Susceptible	Resistant	Resistant
CSK4-PG	AR (mm)	0	1	14	9	12	6	5	4
CSK4-PG	Profile	Resistant	Resistant	Susceptible	Susceptible	Susceptible	Susceptible	Resistant	Resistant
CSK12-PG	AR (mm)	0	0	11	7	9	4	1	1
CSK12-PG	Profile	Resistant	Resistant	Susceptible	Susceptible	Susceptible	Equivocal^b	Resistant	Resistant
CSK7-PG	AR (mm)	0	0	14	10	9	5	3	3
CSK7-PG	Profile	Resistant	Resistant	Susceptible	Susceptible	Susceptible	Susceptible	Resistant	Resistant
CSK8-PG	AR (mm)	0	0	14	9	10	4	1	3
CSK8-PG	Profile	Resistant	Resistant	Susceptible	Susceptible	Susceptible	Equivocal^b	Resistant	Resistant
DWK2-PG	AR (mm)	0	4	14	9	12	5	4	6
DWK2-PG	Profile	Resistant	Equivocal^b	Susceptible	Susceptible	Susceptible	Susceptible	Resistant	Equivocal^b
DWK23-PG	AR (mm)	0	0	9	9	7.5	3	0	3
DWK23-PG	Profile	Resistant	Resistant	Susceptible	Susceptible	Susceptible	Resistant	Resistant	Resistant
DWK8-KP	AR (mm)	0	4	10	8	11	4	9	1
DWK8-KP	Profile	Resistant	Equivocal^b	Susceptible	Susceptible	Susceptible	Equivocal^b	Susceptible	Resistant

Table 4 provides a summary of antibiotic sensitivity results for each pouch-derived bacterial isolate in addition to Pluralibacter gergoviae ATCC33028 and Klebsiella pneumoniae ATCC1388, which were used as controls. Sensitivity testing was performed via disk diffusion in adherence to CDS guidelines for Enterobacteriaceae [45]. For each isolate, the annular radii (AR) of each antibiotic zone of inhibition is reported in millimetres (mm), followed by the resistance profile inferred from said measurements by CDS guidelines. An AR > 6 mm indicates resistance to antibiotics unless marked with ^a, which have AR cutoffs of > 4 mm
^bEquivocal profiles were determined where the AR = cutoff and are generally referred for further testing where possible. AMP25, ampicillin (25 µg); TE10, tetracycline (10 µg); CIP2.5, ciprofloxacin (2.5 µg); CN10, gentamycin (10 µg); CAZ10, ceftazidime (10 µg); AZM15, azithromycin (15 µg); C30, chloramphenicol (30 µg); AMC15, augmentin (15 µg)

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