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Fig. 6 | Microbiome

Fig. 6

From: Inulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium

Fig. 6

Cytokine IL-22 production is enhanced by inulin diet and is crucial for the epithelial proliferative phenotype. A Relative mRNA expression of Il22, Rorc, Ahr and Il17 of colonic lamina propria lymphocytes by RT-qPCR (n = 6). B-D, F Flow cytometry analyses of colonic lamina propria immune cells (n = 4–6). B Quantification of ILC3s. C Gating strategy to define IL-22-positive events within the ILC3 population. D Quantification of the percentage of IL-22-positive ILC3s. E Heatmap with relative mRNA expression of IL-22-target genes of colonic epithelial cells by RT-qPCR (n = 6). F Quantification of the percentage of ILC3s (left) and IL-22-positive ILC3s (right). Results analyzed by two-way ANOVA. Related to fecal microbiota transplanted (MBT) mice described in Fig. 5C (n = 4–8). G Schematic of the experimental model with IL22 KO mice and the different diets (n = 10–12). H Quantification of colon crypt depth (left) and number of EdU-positive cells per crypt (right) of IL22 KO mice. Data were pooled from 2 independent experiments. Results analyzed by Mann–Whitney test. In all graphs, each point represents an individual animal. Unless otherwise stated, results were analyzed by Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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