Fig. 2
From: Inulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium
Ingestion of inulin enhances the proliferative activity of colonic Lgr5 + stem cells. A Experimental model scheme with reporter mice and two dietary groups. B Quantification of the percentage of Lgr5-GFP-positive cells in the colon by flow cytometry (n = 7). C Quantification of the percentage of Lgr5-GFP-positive crypts in the colon by immunohistochemistry (n = 5). D Experimental model scheme with lineage-tracer mice and long-term (3 days) tamoxifen injection. E Visualization of tdTomato-positive cells in colonic crypts by optical microscopy following staining with anti-tdTomato antibody. Scale bars, 100 µm. F Quantification of the ratio of tdTomato-positive length relative to crypt length (n = 4). In all graphs, each point represents an individual animal. Results were analyzed by Student’s t-test. *p < 0.05, and ns = not significant