Fig. 2

Rhizosphere amplicon sequence variants (ASVs) responsible for the community differences in the wild wheats and domesticated wheats that are calculated by a differential abundance test and random forest classification. A and B The volcano plot illustrating the enrichment and depletion patterns of rhizosphere bacterial and fungal microbiomes in the three wild wheats compared with three domesticated wheat accessions. The ASVs were colored by their categorization as “wild enriched,” “domesticated enriched,” and “non-differential” according to their values of Log2 (count per million) and Log2 (fold change). DI, depleted index; DSI, dissimilarity index. C and D Joyplots showing the relative abundance profiles of top 20 ASVs in bacterial and fungal communities that are revealed by a random forest (RF) classifier. The tenfold validation was performed to evaluate the accuracy of RF model and to select minimum number of ASVs with the lowest prediction error rate. The top ASVs on the genus level are listed along the y-axis representing their importance in contributing to the accuracy of domesticated and wild wheats prediction by calculating their mean decrease accuracy in the RF model