Fig. 3

Fecal microbiomes of transition cows with varying lipolysis status profiled using metagenomic sequencing. A Comparison of microbial domains between LNF and HNF cows. Significantly different domains were tested by Wilcoxon rank-sum. Data is presented as mean ± SEM. B Bacterial community profiles of LNF and HNF fecal samples at species level visualized using principal-coordinate analysis and Bray–Curtis distance matrix. C Abundance of significantly different bacterial species between LNF and HNF. Bar plots represent mean ± SEM. D Abundance of significantly different archaea species between LNF and HNF. Bar plots represent mean ± SEM. E Changes between the two co-occurrence networks corresponding to LNF and HNF captured using NetShift which reflected.by the neighbor shift (NESH) cores. Node size shows the predicated “driver” scores; the big, red nodes are particularly important drivers. Edge connections in green are presented only in LNF cows, red is only in HNF cows