Fig. 1
IL-22Fc consistently alters microbiota composition along the GI tract in mice. A Shannon’s diversity following IL-22Fc or aRW treatment. B NMDS ordination plot of Bray-Curtis distances. Dashed ellipses represent the 95% CI for the centroid of each stratification group. r2 calculated by PERMANOVA. For A and B, triangle, square, and circle markers indicate ileal, colonic, and fecal samples, respectively. Teal and red markers indicate animals treated with IL-22Fc (n=8) or isotype control (n=8), respectively. C Taxonomic tree depicting all bacterial genera detected within the ileum, colon, or stool of IL-22Fc or aRW-treated mice. The outer heatmap displays the difference in mean, normalized abundance of a given bacterial genera between IL-22Fc and aRW-treated mice (Log2(∆)) in the ileum, colon, or stool. All non-zero Log2(∆) values have a BH-adjusted p value <0.05. Highly IL-22Fc responsive genera, defined as the top ten genera based on average Log2(∆) and significant enrichment or depletion in IL-22Fc-treated animals across multiple tissues, are labeled. Note that only seven IL-22Fc-enriched genera met these criteria