Fig. 3From: New insights into the impact of microbiome on horizontal and vertical transmission of a tick-borne pathogenA The fold change of rickettsial loads in F1 or F2 generation relative to F0 female ticks during vertical transmission, which was calculated by the 2−ΔΔCt method and F0 females as control (dash line) **P < 0.01, ***P < 0.001. B FISH detection of R. raoultii in different organs. Nuclei, DAPI (blue), R. raoultii: Quasar570 (red)Back to article page