Fig. 6

Visualization of nitrogen-fixing bacteria and root-knot nematodes in tomato roots. A, B FISH protocol fluorescence signals indicated the distribution of nitrogen-fixing bacteria in tomato root tissues by labeling the nitrogenase gene (nifH). C–E Fluorescence signals from nitrogen-fixing bacteria associated with root-knot nematode juveniles (C) or adult females (D, E) using the nifH probe in tomato root tissues. Arrows indicated nematode juveniles (C) or female adults (D, E). Scale bar = 100 μm