Table 1 Description of the removal treatments applied to soil suspension in Step 1 and the corresponding abbreviations used in figures and text
Treatment | Abbreviation | Description |
|---|---|---|
Control | C | Not treated soil suspension |
Ciprofloxacin | Cip | Antibiotic, 66.67 μg/ml for 5h; wash (×3) |
Gentamicin | Gen | Antibiotic, 69.44 μg/ml for 5h; wash (×3) |
Ramoplanin | Ram | Antibiotic, 69.44 μg/ml for 5h; wash (×3) |
Ciclopirox | Cic | Fungicide, 200 μg/ml for 5h; wash (×3) |
Micafungin | Mic | Fungicide, 66.67 μg/ml for 5h; wash (×3) |
Miltefosin | Mil | Protisticide, 69.44 μg/ml for 5h; wash (×3) |
Filtration 1 | F1 | Fraction > 5 μm (5-μm filter) |
Filtration 2 | F2 | 5 μm > fraction > 3 μm (3-μm filter) |
Filtration 3 | F3 | 3 μm > fraction > 1 μm (1-μm filter) |
Freeze-Thaw | FT | 6 x (15 min at −80°C then 15 min at +30°C) |
Heat shock | HS | 0°C for 5 min/70°C for 15 min/0°C for 5 min |
Osmotic | Na | NaCl, 0.1 g/ml for 2 h; wash (×3) |
Oxidative 1 | Ox1 | H2O2 50 mM for 2 h; wash (×3) |
Oxidative 2 | Ox2 | H2O2 25 mM for 2 h; wash (×3) |
Alkaline | pH11 | 0.5 mL ammoniac 20% at 1 M for 2 h; wash (×3) |
Acid | pH2 | 1 mL malic acid at 1 M for 2 h; wash (×3) |
Sonication | US | 9 cycles of 30 s ultrasounds (Vibracell VC-500, 20 kHz) and 30 s rest |
UV | UV | 2-h exposure |