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Fig. 8 | Microbiome

Fig. 8

From: Core-predominant gut fungus Kazachstania slooffiae promotes intestinal epithelial glycolysis via lysine desuccinylation in pigs

Fig. 8

K. slooffiae promotes intestinal epithelial glycolysis in vivo. A, B Representative western blots of lysine-succinylated proteins and β-tubulin in jejunal epithelial cells of GF mice using a pan-lysine succinylation antibody and an anti-β-tubulin antibody, respectively (Ctrl, Control; KS, K. slooffiae) (A). Quantification of lysine-succinylated proteins levels normalized to β-tubulin levels (B). C–E Glucose consumption (C), lactate production (D), and ATP content (E) in jejunal epithelial cells of GF mice. F Body weights of GF mice. G, H Representative western blots of lysine-succinylated proteins and β-tubulin in jejunal epithelial cells of piglets using a pan-lysine succinylation antibody and an anti-β-tubulin antibody, respectively (G). Quantification of lysine-succinylated proteins levels normalized to β-tubulin levels (H). I–K Glucose consumption (I), lactate production (J), and ATP content (K) in the jejunal epithelial cells of piglets. L Body weights of piglets. M Representative images of intestinal histological morphology by H&E staining. N–P Statistical analysis of villus height (N), crypt depth (O), and the ratio of the villus height to crypt depth (P). Data are presented as mean ± SEM and evaluated using Student’s t-test in B–F and H–L. Data are presented as mean ± SEM and evaluated by two-way ANOVA with adjustment for multiple comparisons in N–P; n = 10 (Ctrl group in C–F), n = 8 (KS group in C–F), n = 10 (I–L), n = 5 (N–P), n = 3 (B and H). **p < 0.01, *p < 0.05, ns, not significant

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