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Fig. 4 | Microbiome

Fig. 4

From: Core-predominant gut fungus Kazachstania slooffiae promotes intestinal epithelial glycolysis via lysine desuccinylation in pigs

Fig. 4

K. slooffiae decreases lysine succinylation levels in IPEC-J2 cells. A–C OD600nm of fungal culture suspensions after culturing for 8 h (A), 12 h (B), and 24 h (C), respectively. D Coomassie blue staining of protein samples from whole-cell lysates of IPEC-J2 cells. E–G Representative western blots of lysine-succinylated proteins and β-tubulin in IPEC-J2 cells using a pan anti-succinyllysine monoclonal antibody and an anti-β-tubulin antibody, respectively (E and F). G Quantification of lysine-succinylated proteins levels normalized to β-tubulin levels from the blots shown in F. H, I Analysis of the lysine succinylome based on PCA (H) and Pearson’s correlation coefficient (I). J Statistical analysis of differential proteins and sites with lysine succinylation comparing the K. slooffiae (KS) group with the control (Ctrl) group. Up indicates that lysine succinylation levels of the proteins or sites in KS group were higher than those in Ctrl group. Down indicates that lysine succinylation levels of the proteins or sites in KS group were lower than those in Ctrl group. K Volcano plots showing differential sites with lysine succinylation comparing the KS group with the Ctrl group. Data are shown as mean ± SEM and evaluated by one-way analysis of variance (ANOVA) with adjustment for multiple comparisons in A–C (n = 4). Data are presented as the mean ± SEM and were evaluated by two-way ANOVA with adjustment for multiple comparisons in G (n = 3). **p < 0.01. A ratio of 1.5-fold (>1.50 or <0.67) with a p-value < 0.05 was chosen as the cutoff for up- or downregulation in lysine succinylation in J and K (n = 3)

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