Fig. 6

Amplicon sequencing of the genetic diversity of the GP075 variant genes. GP075 was composed of 243 amino acid residues and their sequence positions were labeled by the natural numbers on the x-axes. The single letters (not including that of Fig. 6e) represented the amino acids. a Percent of the polar amino acid substitutions in the GP075 variants. b Percent of the charged amino acid substitutions in the GP075 variants. c Percent of the hydrophobic amino acid substitutions in the GP075 variants. d Percent of the stop codons in the GP075 variants. e. Percent of the duplications in the GP075 variants, D7, D14, and D21. Duplications were located between the 113th and 114th amino acid residues of GP075, D7 with one additional copy of the 7-aa peptide (APWYSV), D14 with 2 additional contiguous copies of the 7-aa peptide, and D21 with 3 additional contiguous copies of the 7-aa peptide. f Percent of the amino acid substitutions of GP075 identified in the isolated K8-T239A-like mutants. The positions of the amino acid substitutions of GP075 were labeled on the x-axis. Color bars represented the percent of the amino acid substitutions at the specific sites of the GP075 variants revealed by amplicon sequencing of the pool of 2000 phage plaques collected from the bacterial lawns of the OSA-defective strains. Mutations with the percentage value less than 0.02% (dots within the blue rectangles) were considered as experimental noise generated from the analysis. Mutants: the K8-T239A-like mutants isolated from the single phage plaques formed on the lawns of the OSA defective strains