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Fig. 1 | Microbiome

Fig. 1

From: Variants of a putative baseplate wedge protein extend the host range of Pseudomonas phage K8

Fig. 1

Screening of phage mutants infecting OSA-loss cells. a Host range analysis of phage K8 by spotting assay. Indicated amounts of phage K8 were spotted on the bacterial lawns of the indicated strains. Light grey zones represent bacterial lawns. Dark grey zones or dots represent transparent zones or plaques formed in phage infections. b Frequency determination of the K8 mutants, which can lyse the OSA-defective strains SK2, SK15, and SK45, in the K8 population. The experiments were independently repeated 12 times. c Host range analysis of the mutant phage K8-T239A by spotting assay. Indicated amounts of the mutant phage K8-T239A were spotted on the bacterial lawns of the indicated strains. Light grey zones represent bacterial lawns. Dark grey zones or dots represent transparent zones or plaques formed in phage infections. d Frequency determination of the resistant cells. The spontaneous mutation frequencies of the wild-type strain PAK and the OSA-defective strain SK75 were measured when treated with phage K8 and K8-T239A, respectively. The experiments were independently repeated 10 times. One-way ANOVA was performed followed by a Tamhane T2 test to compare the means of the three data groups. ***P < 0.001

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