Fig. 8

A functional Mcr-1 is required to induce (i) LL-37 resistance, (ii) modulation of pro-inflammatory properties of LPS, and (iii) increase bacterial adhesion to eukaryotic cells. E. coli CFT073 were transformed with wild-type mcr-1 (WT mcr-1) or inactivated mcr-1 (Inactivated mcr-1). A 2.10⁷E. coli WT mcr-1⁺ or 2.10⁷E. coli Inactivated mcr-1^- were incubated for 2 h with 0.2 μg of LL-37. The percentage of surviving bacteria was reported. Data are the means ± SEMs of four replicates and are representative of six independent experiments. B, D HT-29 cells were infected at an MOI of 100 for 3 h with E. coli WT mcr-1⁺ or with E. coli Inactivated mcr-1^-. C HT-29 cells were stimulated for 3 h with LPS (10 μg/ml) purified from E. coli WT mcr-1⁺ or E. coli Inactivated mcr-1^-. B, C The amounts of secreted IL-8 in the cell culture supernatant were quantified by ELISA. Data are the means ± SEMs of six replicates and are representative of two independent experiments. D Adherent bacteria were counted, and the data are expressed as the number of colony-forming units (CFUs)/well. Data are the means ± SEMs of six replicates and are representative of two independent experiments