Fig. 7
Expression of Mcr-1 by commensal E. coli HS strain, induces resistance to LL-37, decreases LPS pro-inflammatory response and increases bacterial adhesion to eukaryotic cells. E. coli HS were transformed with empty pBK-CMV (E. coli mcr-1-) or with pBK-CMV-mcr-1 (E. coli mcr-1+). A 2.107E. coli mcr-1+ or 2.107E. coli mcr-1- were incubated for 1.30 h with 0.1 μg of LL-37. The percentage of surviving bacteria was reported. Data are the means ± SEMs of four replicates and are representative of six independent experiments. B HT-29 cells were stimulated for 3 h with LPS (10 μg/ml) purified from E. coli mcr-1+ or E. coli mcr-1-. The amounts of secreted IL-8 in the cell culture supernatant were quantified by ELISA. Data are the means ± SEMs of six replicates and are representative of two independent experiments. C HT-29 cells were infected for 3 h with E. coli mcr-1+ or with E. coli mcr-1-. Adherent bacteria were counted, and the data are expressed as the number of colony-forming units (CFUs)/well. Data are the means ± SEMs of six replicates and are representative of two independent experiments