Fig. 6

mcr-1 increases bacterial adherence to eukaryotic cells. A HT-29 cells and B THP-1 cells were infected for 3 h and 1 h, respectively, with E. coli mcr-1⁺ or with E. coli mcr-1^-. Adherent bacteria were counted, and the data are expressed as the number of colony-forming units (CFUs)/well. Data are the means ± SEMs of six replicates and are representative of three independent experiments. C, D HT29-16E cells were infected E. coli mcr-1⁺ or with E. coli mcr-1^- and stained for bacteria (red), mucus (green), and nuclei (blue). C Representative images are shown. D Quantification of bacteria per fields. Data are the means ± SEMs of 26 fields and are representative of two independent experiments