Fig. 1
mcr-1 enhances gut colonization in the oligo-mouse-microbiota 12 (OMM12) model without drastically affecting gut microbiota composition. Gnotobiotic OMM12 mice were orally infected with E. coli mcr-1+ or with E. coli mcr-1-, and intestinal colonization was assessed at day 10 post-infection. A The relative abundances of OMM12 strains were determined by a strain-specific qPCR assay and are plotted as the relative abundances (expressed as the fraction of total 16S rRNA gene copy numbers). YL44, Akkermansia muciniphila; I48, Bacteroides caecimuris; YL27, Muribaculum intestinale; YL45, Turicimonas muris; YL2, Bifidobacterium longum; KB1, Enterococcus faecalis; KB18, Acutalibacter muris; YL32, Clostridium clostridioforme; YL31, Flavonifractor plautii; YL58, Blautia coccoides; I49, Lactobacillus reuteri; I46, Clostridium innocuum; and E. coli CFT073. Data are means ± SEMs. B E. coli abundance was assessed in caecum content and faeces by the serial dilution culture method (expressed as CFUs per g of caecum content or faeces) and by E. coli-specific 16S rRNA gene qPCR (expressed as copy numbers per ng of fecal genomic DNA). Data are means ± SEMs