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Fig. 7 | Microbiome

Fig. 7

From: Ultra-sensitive isotope probing to quantify activity and substrate assimilation in microbiomes

Fig. 7

Strong differences in heavy water incorporation in intestinal microbiota species in response to diet. Sixty-three species isolated from human intestinal microbiota were grown together in triplicates in either a high fiber or high protein medium in the presence of unlabeled water or water with either 25% 2H or.18O [11]. Calis-p-based stable isotope ratios are shown for the 20 species for which at least 9 peptides passed Calis-p filtering conditions in all replicates. Each box shows the data for all peptides of the triplicate cultures combined (27 to 2225 peptides per box). The red lines indicate the average median for each species in the control samples with unlabeled water. Statistically significant differences are indicated with ‘*’ based on Student’s t test on the means of replicates at p < 0.05

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