Fig. 5

Intervention of mice with microbial communities lacking or including L. reuteri influence the anti-obesity phenotypes of CQA. The mice were fed a HFD for 10 weeks and then gavaged with vehicle or CQA (150 mg/kg) together with selective antibiotic in drinking water for 4 weeks. a Schematic diagram of the selective antibiotic supplementation in combination with CQA. b Body weight. c Serum triglyceride. d Serum T-CHO. e Hepatic triglyceride. f Hepatic T-CHO. g Core temperature. h Relative mRNA expression of thermogenic genes in iWAT. i Representative UCP1 staining of iWAT sections, scale bar: 50 μm. The mice were fed a HFD for 10 weeks and then gavaged with vehicle or CQA (150 mg/kg) together with defined microbiota colonization for 4 weeks. j Composition of the defined microbiota for the respective experimental groups. k Body weight. l Serum triglyceride. m Serum T-CHO. n Hepatic triglyceride. o Hepatic T-CHO. p Core temperature. q Relative mRNA expression of thermogenic genes in iWAT. r Representative UCP1 staining of iWAT sections, scale bar: 50 μm. n = 5/group. Data are presented as mean ± SD. *p < 0.05; **p < 0.01; and ***p < 0.001. ns means not statistically significant