Fig. 2

Comparison of mOTUs to other taxonomic profilers. The performance of mOTUs3 was compared to other taxonomic profiling tools based on the dataset from the second Critical Assessment of Metagenome Interpretation (CAMI) challenge (see Methods). The F1 score (a) and L1 norm error (b) are shown as reported by the OPAL tool [25] for each taxonomic rank (x-axis). High L1 norm error values at the family and genus levels of GI samples mostly derive from an updated taxonomy of the highly abundant Oscillospiraceae (previously Ruminococcaceae) [42]. c Each method was ranked across all samples and for each taxonomic rank using four measures (completeness, purity, L1 norm error and weighted UniFrac error), and the OPAL sum of scores was calculated as a sum of these ranks (lower rank indicates better performance). OR oral cavity, SK skin, AI airways, UT urogenital tract, GI gastrointestinal tract, MG mouse gut