Fig. 7

Aromatic cresols are markers of L. reuteri tryptophan-dependent enhanced autoimmune predisposition. Serum was collected from naïve mice fed a low- or high-tryptophan diet colonized with either the B6 or B6+L. reuteri microbiota and analyzed via UPLC-MS/MS as outlined in Fig. 4A, B. Partial least squares-discriminant analysis (PLS-DA) and volcano plots of differentially abundant metabolites, passing a threshold of |FoldChange|>1.5 and P<0.05 in low-tryptophan-fed mice (A, B) or high-tryptophan-fed mice (C, D). Post hoc analysis using Fisher’s LSD of aromatic cresols in serum (E) or L. reuteri monoculture (F) are represented as log-transformed and mean centered abundance plots