Question | Statistical approach | Response | Predictor(s) | Random effect |
---|---|---|---|---|
1) How closely does the microbiome composition of the worm resemble that of its immediate environment, the jejunum? | GLMM | Pig-Ascaris Bray-Curtis dissimilarity | Same host (yes, no) Location (jejunum, other compartment) Location: same host (interaction) | Individuals (pig ID and Ascaris ID) |
2) How either host or parasite origin determine the composition of their microbiomes when only dominant microbes are taken into account? | PERMANOVA | Jejunum-Ascaris BC dissimilarity | Origin (host or parasite) Individual host (pig IDs) | - |
3) What is the impact of the host, worm sex, and dominant bacteria on microbiome variation from pigs and Ascaris? | PERMANOVA | Pig-Ascaris BC dissimilarity | Host (pig IDs) Worm sex (male, female) Dominant bacteria (Clostridium sensu stricto 1 Lactobacillus, Escherichia-Shigella, Prevotella, Streptococcus, and Romboutsia) | - |
4) What is the impact of the host of origin and worm sex on microbiome variation among Ascaris individuals? | GLMM | Ascaris-Ascaris BC dissimilarity | Same host (yes, no) Same sex (yes, no) | Individuals (Ascaris IDs) |
5) Is the infection status a relevant factor driving the differences in microbial composition between host and parasites? | GLMM | Pig-pig BC dissimilarity | Same host (yes, no) Same compartment (yes, no) Same infection status (yes, no) | Individuals (pig IDs) |