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Table 1 Statistical modeling

From: Guts within guts: the microbiome of the intestinal helminth parasite Ascaris suum is derived but distinct from its host

Question

Statistical approach

Response

Predictor(s)

Random effect

1) How closely does the microbiome composition of the worm resemble that of its immediate environment, the jejunum?

GLMM

Pig-Ascaris Bray-Curtis dissimilarity

Same host (yes, no)

Location (jejunum, other compartment)

Location: same host (interaction)

Individuals (pig ID and Ascaris ID)

2) How either host or parasite origin determine the composition of their microbiomes when only dominant microbes are taken into account?

PERMANOVA

Jejunum-Ascaris BC dissimilarity

Origin (host or parasite)

Individual host (pig IDs)

-

3) What is the impact of the host, worm sex, and dominant bacteria on microbiome variation from pigs and Ascaris?

PERMANOVA

Pig-Ascaris BC dissimilarity

Host (pig IDs)

Worm sex (male, female)

Dominant bacteria (Clostridium sensu stricto 1 Lactobacillus, Escherichia-Shigella, Prevotella, Streptococcus, and Romboutsia)

-

4) What is the impact of the host of origin and worm sex on microbiome variation among Ascaris individuals?

GLMM

Ascaris-Ascaris BC dissimilarity

Same host (yes, no)

Same sex (yes, no)

Individuals (Ascaris IDs)

5) Is the infection status a relevant factor driving the differences in microbial composition between host and parasites?

GLMM

Pig-pig BC dissimilarity

Same host (yes, no)

Same compartment (yes, no)

Same infection status (yes, no)

Individuals (pig IDs)