Fig. 4

Comparison of efficiency of carbon source utilization of mutants harboring glcU gene variations with those without in Phenotype MicroArray analysis on Biolog AN microplates. A Cluster heatmap of area under curve (AUC) of respiration kinetics curves of each substrate of 10 isolates (seven mother-infant pair isolates containing nonsynonymous mutation in the glcU gene: G4M3, G9C4, G7R82, T6M7, T12M12, T16C12, T16C15; and three M8 dry powder-associated isolates without mutation in the glcU gene: M8-1, M8-3, M8-6). Cluster analysis was performed by the unweighted pair group method with arithmatic mean (UPGMA). The color scale represents AUC. A higher value represents a larger AUC. B The respiration kinetics curves of 12 differential metabolic carbon sources of the 10 isolates. Time in hours and the observed metabolic signals in OmniLog units are represented on the x- and y-axes, respectively. C Metabolic differences in the capacity of utilization of 12 carbon sources between mother-infant pair isolates containing mutation in glcU (glcU mutants) and M8 dry powder-associated isolates without mutation in glcU (non-glcU mutants). Data were represented as mean ± SEM. P values were generated by t test